UniProt Accession

 BRAF_HUMAN; P15056; A4D1T4; B6HY61; B6HY62; B6HY63; B6HY64; B6HY65; B6HY66; Q13878; Q3MIN6; Q9UDP8; Q9Y6T3 

Genbank Protein ID

 M95712; AC006344; EU600171; AC006347; CH236950; BC101757; BC112079; X65187; M21001; AM989472; AM989473; AM989474; AM989475; AM989476; AM989477 

Genbank Nucleotide ID

 AAA35609.2; AAD43193.1; ACD11489.1; AAD15551.1; EAL24023.1; AAI01758.1; AAI12080.1; CAA46301.1; AAA96495.1; CAQ43111.1; CAQ43112.1; CAQ43113.1; CAQ43114.1; CAQ43115.1; CAQ43116.1 

Protein Name

 Serine/threonine-protein kinase B-raf 

Protein Synonyms/Alias

 Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1 

Gene Name

 BRAF 

Gene Synonyms/Alias

 BRAF1; RAFB1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
88	AYEEYTSKLDALQQR	ubiquitination	[1, 2]
164	VRVFLPNKQRTVVPA	ubiquitination	[3]
253	AFCDFCRKLLFQGFR	ubiquitination	[4]
418	TNVKALQKSPGPQRE	acetylation	[5]
473	GSFGTVYKGKWHGDV	ubiquitination	[3]
601	DFGLATVKSRWSGSH	ubiquitination	[3]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 

Functional Description

 Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. 

Sequence Annotation

 DOMAIN 155 227 RBD.
 DOMAIN 457 717 Protein kinase.
 ZN_FING 234 280 Phorbol-ester/DAG-type.
 NP_BIND 463 471 ATP (By similarity).
 ACT_SITE 576 576 Proton acceptor (By similarity).
 METAL 235 235 Zinc 1 (By similarity).
 METAL 248 248 Zinc 2 (By similarity).
 METAL 251 251 Zinc 2 (By similarity).
 METAL 261 261 Zinc 1 (By similarity).
 METAL 264 264 Zinc 1 (By similarity).
 METAL 269 269 Zinc 2 (By similarity).
 METAL 272 272 Zinc 2 (By similarity).
 METAL 280 280 Zinc 1 (By similarity).
 BINDING 483 483 ATP (By similarity).
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 365 365 Phosphoserine; by SGK1.
 MOD_RES 373 373 Phosphothreonine; by autocatalysis.
 MOD_RES 396 396 Phosphothreonine.
 MOD_RES 399 399 Phosphoserine.
 MOD_RES 401 401 Phosphothreonine.
 MOD_RES 446 446 Phosphoserine.
 MOD_RES 447 447 Phosphoserine.
 MOD_RES 671 671 Omega-N-methylarginine; by PRMT5.
 MOD_RES 729 729 Phosphoserine.
 MOD_RES 753 753 Phosphothreonine; by MAPK1.  

Keyword

 3D-structure; Acetylation; ATP-binding; Cardiomyopathy; Cell membrane; Chromosomal rearrangement; Complete proteome; Cytoplasm; Deafness; Direct protein sequencing; Disease mutation; Kinase; Membrane; Metal-binding; Methylation; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Proto-oncogene; Reference proteome; Serine/threonine-protein kinase; Transferase; Ubl conjugation; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 766 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005509; F:calcium ion binding; IDA:BHF-UCL.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
 GO:0005057; F:receptor signaling protein activity; IEA:InterPro.
 GO:0000186; P:activation of MAPKK activity; TAS:Reactome.
 GO:0071277; P:cellular response to calcium ion; IDA:BHF-UCL.
 GO:0008543; P:fibroblast growth factor receptor signaling pathway; TAS:Reactome.
 GO:0043066; P:negative regulation of apoptotic process; IDA:UniProtKB.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0009887; P:organ morphogenesis; TAS:ProtInc.
 GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; IDA:BHF-UCL.
 GO:0010628; P:positive regulation of gene expression; IMP:BHF-UCL.
 GO:0033138; P:positive regulation of peptidyl-serine phosphorylation; IDA:UniProtKB.
 GO:0070849; P:response to epidermal growth factor stimulus; IDA:BHF-UCL.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:Reactome.
 GO:0007268; P:synaptic transmission; TAS:Reactome. 

Interpro

 IPR020454; DAG/PE-bd.
 IPR011009; Kinase-like_dom.
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR003116; Raf-like_ras-bd.
 IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF00130; C1_1
 PF07714; Pkinase_Tyr
 PF02196; RBD 

SMART

 SM00109; C1
 SM00455; RBD 

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST
 PS50898; RBD
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 

PRINTS

 PR00008; DAGPEDOMAIN.