CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-044461
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Serine hydroxymethyltransferase, cytosolic 
Protein Synonyms/Alias
  
Gene Name
 SHMT1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
10MPVNGAHKDADLWSSubiquitination[1]
27KMLAQPLKDSDVEVYubiquitination[1, 2]
38VEVYNIIKKESNRQRubiquitination[2, 3]
39EVYNIIKKESNRQRVubiquitination[1]
72LGSCLNNKYSEGYPGubiquitination[1, 4, 5, 6]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 128 AA 
Protein Sequence
MTMPVNGAHK DADLWSSHDK MLAQPLKDSD VEVYNIIKKE SNRQRVGLEL IASENFASRA 60
VLEALGSCLN NKYSEGYPGQ RLPCKLCCVH CPGGTPWAHH GPGPSGWGPP DPWVHDRQEE 120
NLCHVHLL 128 
Gene Ontology
 GO:0004372; F:glycine hydroxymethyltransferase activity; IEA:InterPro.
 GO:0008242; F:omega peptidase activity; IEA:InterPro.
 GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
 GO:0006544; P:glycine metabolic process; IEA:InterPro.
 GO:0006563; P:L-serine metabolic process; IEA:InterPro. 
Interpro
 IPR006155; Josephin.
 IPR015424; PyrdxlP-dep_Trfase.
 IPR015421; PyrdxlP-dep_Trfase_major_sub1.
 IPR001085; Ser_HO-MeTrfase. 
Pfam
 PF00464; SHMT 
SMART
  
PROSITE
 PS50957; JOSEPHIN 
PRINTS