CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024259
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Threonine synthase 
Protein Synonyms/Alias
 TS 
Gene Name
 thrC 
Gene Synonyms/Alias
 MSMEG_4956; MSMEI_4829 
Created Date
 July 27, 2013 
Organism
 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 
NCBI Taxa ID
 246196 
Lysine Modification
Position
Peptide
Type
References
151DCLELARKLTADFPTpupylation[1, 2]
Reference
 [1] Expansion of the mycobacterial "PUPylome".
 Watrous J, Burns K, Liu WT, Patel A, Hook V, Bafna V, Barry CE 3rd, Bark S, Dorrestein PC.
 Mol Biosyst. 2010 Feb;6(2):376-85. [PMID: 20094657]
 [2] Proteome-wide identification of mycobacterial pupylation targets.
 Poulsen C, Akhter Y, Jeon AH, Schmitt-Ulms G, Meyer HE, Stefanski A, Stühler K, Wilmanns M, Song YH.
 Mol Syst Biol. 2010 Jul 13;6:386. [PMID: 20631680] 
Functional Description
 Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine (By similarity). 
Sequence Annotation
 REGION 196 200 Pyridoxal phosphate binding (By
 BINDING 95 95 Pyridoxal phosphate (By similarity).
 BINDING 326 326 Pyridoxal phosphate (By similarity).
 MOD_RES 69 69 N6-(pyridoxal phosphate)lysine (By
 CROSSLNK 151 151 Isoglutamyl lysine isopeptide (Lys-Gln)  
Keyword
 Amino-acid biosynthesis; Complete proteome; Isopeptide bond; Lyase; Pyridoxal phosphate; Reference proteome; Threonine biosynthesis; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 360 AA 
Protein Sequence
MSAAKAAVHQ PWPGLIEAYR DRLPIGDDWT TVTLLEGGTP LIHAKRISEL TGCTVHLKVE 60
GLNPTGSFKD RGMTVAVTES LARGQQAVLC ASTGNTSASA AAYAARAGIT CAVLIPQGKI 120
AMGKLAQAVM HGAKIIQVDG NFDDCLELAR KLTADFPTIA LVNSVNPYRI EGQKTAAFEI 180
VDALGTAPDV HALPVGNAGN ITAYWKGYSE YHRDGVSDRL PRMLGTQAAG AAPLVTGAPV 240
KDPETIATAI RIGSPASWNS AVEAQQQSDG RFLAATDEEI LAAYHLVART EGVFVEPASA 300
ASIAGLLKSV EDGWVKRGST VVCTVTGNGL KDPDTALKGM PQVTPVPVDP SAVVAELGLS 360 
Gene Ontology
 GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
 GO:0004795; F:threonine synthase activity; IEA:EC.
 GO:0009088; P:threonine biosynthetic process; IEA:UniProtKB-UniPathway. 
Interpro
 IPR000634; Ser/Thr_deHydtase_PyrdxlP-BS.
 IPR026260; Thr_Synthase_Gram_pos_bac.
 IPR004450; Thr_synthase_like.
 IPR001926; Trp_syn_b_sub_like_PLP_eny_SF. 
Pfam
 PF00291; PALP 
SMART
  
PROSITE
 PS00165; DEHYDRATASE_SER_THR 
PRINTS