UniProt Accession

 YPT1_YEAST; P01123; D6VTJ2 

Genbank Protein ID

 X00209; D50617; AY558467; BK006940 

Genbank Nucleotide ID

 CAA25036.1; BAA09201.1; AAS56793.1; DAA12402.1 

Protein Name

 GTP-binding protein YPT1 

Protein Synonyms/Alias

 Protein YP2; Rab GTPase YPT1; Transport GTPase YPT1 

Gene Name

 YPT1 

Gene Synonyms/Alias

 YP2; YFL038C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
48	IGVDFKIKTVELDGK	ubiquitination	[1]
55	KTVELDGKTVKLQIW	ubiquitination	[1]
122	LKLLVGNKCDLKDKR	ubiquitination	[1]
137	VVEYDVAKEFADANK	ubiquitination	[1]
144	KEFADANKMPFLETS	ubiquitination	[1, 2]
170	LTMARQIKESMSQQN	ubiquitination	[1]
184	NLNETTQKKEDKGNV	ubiquitination	[1]
188	TTQKKEDKGNVNLKG	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

 Involved in the trafficking of secretory vesicles from the endoplasmic reticulum (ER) to the Golgi. Regulates correct targeting and tethering of vesicles to target membranes by catalyzing the selective recruitment of proteins required for tethering and fusion onto membranes. Vesicular transport depends on shuttling of YPT1 between membrane and cytosol by GDI1, probably by recycling it to its membrane of origin after a vesicle fusion event. Required for sorting and transport of proteins from the ER through the Golgi compartment. Also involved in the recycling of membrane proteins. 

Sequence Annotation

 NP_BIND 15 22 GTP (By similarity).
 NP_BIND 63 67 GTP (By similarity).
 NP_BIND 121 124 GTP (By similarity).
 REGION 63 80 Interaction with GDI1.
 REGION 189 195 Interaction with GDI1.
 MOTIF 37 45 Effector region (Probable).
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 172 172 Phosphoserine.
 MOD_RES 174 174 Phosphoserine.
 LIPID 205 205 S-geranylgeranyl cysteine.
 LIPID 206 206 S-geranylgeranyl cysteine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Endoplasmic reticulum; ER-Golgi transport; Golgi apparatus; GTP-binding; Lipoprotein; Membrane; Nucleotide-binding; Phosphoprotein; Prenylation; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 206 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; IDA:SGD.
 GO:0000139; C:Golgi membrane; IDA:SGD.
 GO:0005795; C:Golgi stack; IDA:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0000407; C:pre-autophagosomal structure; IDA:SGD.
 GO:0005802; C:trans-Golgi network; IDA:SGD.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IDA:SGD.
 GO:0000149; F:SNARE binding; IDA:SGD.
 GO:0090114; P:COPII-coated vesicle budding; IMP:SGD.
 GO:0032258; P:CVT pathway; IMP:SGD.
 GO:0034498; P:early endosome to Golgi transport; IMP:SGD.
 GO:0032456; P:endocytic recycling; IMP:SGD.
 GO:0048194; P:Golgi vesicle budding; IGI:SGD.
 GO:0048211; P:Golgi vesicle docking; IMP:SGD.
 GO:0016236; P:macroautophagy; IMP:SGD.
 GO:0006461; P:protein complex assembly; IDA:SGD.
 GO:0006890; P:retrograde vesicle-mediated transport, Golgi to ER; IMP:SGD.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
 GO:0035494; P:SNARE complex disassembly; IMP:SGD. 

Interpro

 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR001806; Small_GTPase.
 IPR003579; Small_GTPase_Rab_type. 

Pfam

 PF00071; Ras 

SMART

 SM00175; RAB 

PROSITE

 PS51419; RAB 

PRINTS

 PR00449; RASTRNSFRMNG.