CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007568
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Caspase-3 
Protein Synonyms/Alias
 CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1; Caspase-3 subunit p17; Caspase-3 subunit p12 
Gene Name
 CASP3 
Gene Synonyms/Alias
 CPP32 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
11TENSVDSKSIKNLEPubiquitination[1]
14SVDSKSIKNLEPKIIubiquitination[1]
57INNKNFHKSTGMTSRubiquitination[1]
82RETFRNLKYEVRNKNacetylation[2]
82RETFRNLKYEVRNKNubiquitination[1, 3, 4]
138NGPVDLKKITNFFRGubiquitination[1]
210YYSWRNSKDGSWFIQubiquitination[1]
229MLKQYADKLEFMHILubiquitination[1, 3, 4, 5]
260DATFHAKKQIPCIVSubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. 
Sequence Annotation
 ACT_SITE 121 121 By similarity.
 ACT_SITE 163 163 By similarity.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 26 26 Phosphoserine (By similarity).
 MOD_RES 163 163 S-nitrosocysteine; in inhibited form.  
Keyword
 3D-structure; Acetylation; Apoptosis; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Phosphoprotein; Polymorphism; Protease; Reference proteome; S-nitrosylation; Thiol protease; Zymogen. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 277 AA 
Protein Sequence
MENTENSVDS KSIKNLEPKI IHGSESMDSG ISLDNSYKMD YPEMGLCIII NNKNFHKSTG 60
MTSRSGTDVD AANLRETFRN LKYEVRNKND LTREEIVELM RDVSKEDHSK RSSFVCVLLS 120
HGEEGIIFGT NGPVDLKKIT NFFRGDRCRS LTGKPKLFII QACRGTELDC GIETDSGVDD 180
DMACHKIPVE ADFLYAYSTA PGYYSWRNSK DGSWFIQSLC AMLKQYADKL EFMHILTRVN 240
RKVATEFESF SFDATFHAKK QIPCIVSMLT KELYFYH 277 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0004190; F:aspartic-type endopeptidase activity; EXP:Reactome.
 GO:0004861; F:cyclin-dependent protein serine/threonine kinase inhibitor activity; IEA:Compara.
 GO:0004197; F:cysteine-type endopeptidase activity; IDA:UniProtKB.
 GO:0008635; P:activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c; TAS:Reactome.
 GO:0006309; P:apoptotic DNA fragmentation; TAS:Reactome.
 GO:0001782; P:B cell homeostasis; IEA:Compara.
 GO:0045165; P:cell fate commitment; IEA:Compara.
 GO:0008625; P:extrinsic apoptotic signaling pathway via death domain receptors; IEA:Compara.
 GO:0007507; P:heart development; IEA:Compara.
 GO:0035329; P:hippo signaling cascade; TAS:Reactome.
 GO:0006917; P:induction of apoptosis; TAS:ProtInc.
 GO:0097193; P:intrinsic apoptotic signaling pathway; TAS:Reactome.
 GO:0008631; P:intrinsic apoptotic signaling pathway in response to oxidative stress; IEA:Compara.
 GO:0030216; P:keratinocyte differentiation; IEA:Compara.
 GO:0046007; P:negative regulation of activated T cell proliferation; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; IGI:MGI.
 GO:0030889; P:negative regulation of B cell proliferation; IEA:Compara.
 GO:0045736; P:negative regulation of cyclin-dependent protein serine/threonine kinase activity; IEA:Compara.
 GO:0051402; P:neuron apoptotic process; IEA:Compara.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0030264; P:nuclear fragmentation involved in apoptotic nuclear change; IMP:HGNC.
 GO:0043525; P:positive regulation of neuron apoptotic process; IEA:Compara.
 GO:0016485; P:protein processing; IEA:Compara.
 GO:0006508; P:proteolysis; IDA:UniProtKB.
 GO:0001836; P:release of cytochrome c from mitochondria; IEA:Compara.
 GO:0006974; P:response to DNA damage stimulus; IEA:Compara.
 GO:0034612; P:response to tumor necrosis factor; TAS:BHF-UCL.
 GO:0009411; P:response to UV; IEA:Compara.
 GO:0009611; P:response to wounding; IEA:Compara.
 GO:0007605; P:sensory perception of sound; IEA:Compara.
 GO:0043029; P:T cell homeostasis; IEA:Compara. 
Interpro
 IPR015470; Caspase_3.
 IPR011600; Pept_C14_cat.
 IPR001309; Pept_C14_ICE_p20.
 IPR016129; Pept_C14_ICE_p20_AS.
 IPR002138; Pept_C14_p10.
 IPR002398; Pept_C14_p45.
 IPR015917; Pept_C14_p45_core. 
Pfam
 PF00656; Peptidase_C14 
SMART
 SM00115; CASc 
PROSITE
 PS01122; CASPASE_CYS
 PS01121; CASPASE_HIS
 PS50207; CASPASE_P10
 PS50208; CASPASE_P20 
PRINTS
 PR00376; IL1BCENZYME.