CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020969
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cytochrome P450 4V2 
Protein Synonyms/Alias
  
Gene Name
 Cyp4v2 
Gene Synonyms/Alias
 Cyp4v3 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
116EVILTSSKQIDKSFLubiquitination[1]
120TSSKQIDKSFLYKFLubiquitination[1]
259RDHKRGLKCLHTFTNubiquitination[1]
277AERVKERKAEEDWTGubiquitination[1]
354TNPEVQRKVDQELDEubiquitination[1]
376PVTLEDLKKLKYLDCubiquitination[1]
379LEDLKKLKYLDCVIKubiquitination[1]
386KYLDCVIKETLRVFPubiquitination[1]
412DCEVGGYKVTKGTEAubiquitination[1]
415VGGYKVTKGTEAIIIubiquitination[1]
471PRNCIGQKFAVMEEKacetylation[2]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 
Functional Description
 Catalyzes the omega-hydroxylation of medium-chain saturated fatty acids such as laurate, myristate and palmitate in an NADPH-dependent pathway. The substrate specificity is higher for myristate > laurate > palmitate (C14>C16>C12) (By similarity). 
Sequence Annotation
 METAL 467 467 Iron (heme axial ligand) (By similarity).
 BINDING 329 329 Heme (covalent; via 1 link) (By
 MOD_RES 403 403 Phosphoserine.  
Keyword
 Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 525 AA 
Protein Sequence
MLWLWLGLSG QKLLLWGAAS AVSLAGATIL ISIFPMLVSY ARKWQQMRSI PSVARAYPLV 60
GHALYMKPNN AEFFQQLIYY TEEFRHLPII KLWIGPVPLV ALYKAENVEV ILTSSKQIDK 120
SFLYKFLQPW LGLGLLTSTG SKWRTRRKML TPTFHFTILE NFLDVMNEQA NILVNKLEKH 180
VNQEAFNCFF YITLCALDII CETAMGKNIG AQSNNDSEYV RTVYRMSDMI YRRMKMPWLW 240
FDLWYLVFKE GRDHKRGLKC LHTFTNNVIA ERVKERKAEE DWTGAGRGPI PSKNKRKAFL 300
DLLLSVTDEE GNRLSQEDIR EEVDTFMFEG HDTTAAAINW SLYLLGTNPE VQRKVDQELD 360
EVFGRSHRPV TLEDLKKLKY LDCVIKETLR VFPSVPLFAR SLSEDCEVGG YKVTKGTEAI 420
IIPYALHRDP RYFPDPEEFR PERFFPENSQ GRHPYAYVPF SAGPRNCIGQ KFAVMEEKTI 480
LACILRQFWV ESNQKREELG LAGDLILRPN NGIWIKLKRR HEDDP 525 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0009055; F:electron carrier activity; IEA:InterPro.
 GO:0020037; F:heme binding; IEA:InterPro.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
 GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
 GO:0010430; P:fatty acid omega-oxidation; ISS:UniProtKB. 
Interpro
 IPR001128; Cyt_P450.
 IPR017972; Cyt_P450_CS.
 IPR002401; Cyt_P450_E_grp-I. 
Pfam
 PF00067; p450 
SMART
  
PROSITE
 PS00086; CYTOCHROME_P450 
PRINTS
 PR00463; EP450I.
 PR00385; P450.