UniProt Accession

 PURB_MOUSE; O35295 

Genbank Protein ID

 AF017630; AL646020 

Genbank Nucleotide ID

 AAB71859.1; CAI26011.1 

Protein Name

 Transcriptional activator protein Pur-beta 

Protein Synonyms/Alias

 Purine-rich element-binding protein B; Vascular actin single-stranded DNA-binding factor 2 p44 component 

Gene Name

 Purb 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
63	KRFYLDVKQNAKGRF	ubiquitination	[1]
72	NAKGRFLKIAEVGAG	acetylation	[2]
72	NAKGRFLKIAEVGAG	ubiquitination	[1]
148	RKYYLDLKENQRGRF	ubiquitination	[1]
279	VPFKAWGKFGGAFCR	acetylation	[2, 3, 4]
279	VPFKAWGKFGGAFCR	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 

Functional Description

 Has capacity to bind repeated elements in single- stranded DNA such as the purine-rich single strand of the PUR element located upstream of the MYC gene. Participates in transcriptional and translational regulation of alpha-MHC expression in cardiac myocytes by binding to the purine-rich negative regulatory (PNR) element. Modulates constitutive liver galectin-3 gene transcription by binding to its promoter. May play a role in the dendritic transport of a subset of mRNAs (By similarity). Plays a role in the control of vascular smooth muscle (VSM) alpha-actin gene transcription as repressor in myoblasts and fibroblasts. 

Sequence Annotation

 DNA_BIND 37 263
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 6 6 Phosphoserine.
 MOD_RES 8 8 Phosphoserine.
 MOD_RES 43 43 Phosphothreonine.
 MOD_RES 113 113 Phosphoserine (By similarity).
 MOD_RES 310 310 Phosphoserine (By similarity).
 MOD_RES 316 316 Phosphoserine.  

Keyword

 Acetylation; Complete proteome; Direct protein sequencing; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 324 AA 

Protein Sequence

Gene Ontology

 GO:0005662; C:DNA replication factor A complex; IDA:UniProtKB.
 GO:0003691; F:double-stranded telomeric DNA binding; ISS:UniProtKB.
 GO:0003729; F:mRNA binding; IDA:UniProtKB.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:MGI.
 GO:0003697; F:single-stranded DNA binding; IDA:UniProtKB.
 GO:0046332; F:SMAD binding; IDA:MGI.
 GO:0006915; P:apoptotic process; NAS:UniProtKB.
 GO:0030154; P:cell differentiation; NAS:UniProtKB.
 GO:0008283; P:cell proliferation; NAS:UniProtKB.
 GO:0006268; P:DNA unwinding involved in replication; ISS:UniProtKB.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:MGI.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR006628; PUR_DNA_RNA-bd. 

Pfam

 PF04845; PurA 

SMART

 SM00712; PUR 

PROSITE

  

PRINTS