CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013454
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Kelch-like protein 22 
Protein Synonyms/Alias
  
Gene Name
 KLHL22 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
195YRQLPLEKVYSLLSSubiquitination[1]
260VLQRLHDKLDPSPLRubiquitination[1]
319TVLSDQAKYLNPLLGubiquitination[1]
329NPLLGEWKHFTASLAubiquitination[1]
433WAYVAPLKREVYAHAubiquitination[1, 2, 3, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation. 
Sequence Annotation
 DOMAIN 50 117 BTB.
 REPEAT 299 349 Kelch 1.
 REPEAT 350 399 Kelch 2.
 REPEAT 400 446 Kelch 3.
 REPEAT 448 493 Kelch 4.
 REPEAT 494 544 Kelch 5.
 REPEAT 545 593 Kelch 6.
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 463 463 Phosphothreonine (By similarity).
 MOD_RES 466 466 Phosphotyrosine (By similarity).
 MOD_RES 475 475 Phosphothreonine (By similarity).  
Keyword
 Acetylation; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Kelch repeat; Mitosis; Phosphoprotein; Reference proteome; Repeat; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 634 AA 
Protein Sequence
MAEEQEFTQL CKLPAQPSHP HCVNNTYRSA QHSQALLRGL LALRDSGILF DVVLVVEGRH 60
IEAHRILLAA SCDYFRGMFA GGLKEMEQEE VLIHGVSYNA MCQILHFIYT SELELSLSNV 120
QETLVAACQL QIPEIIHFCC DFLMSWVDEE NILDVYRLAE LFDLSRLTEQ LDTYILKNFV 180
AFSRTDKYRQ LPLEKVYSLL SSNRLEVSCE TEVYEGALLY HYSLEQVQAD QISLHEPPKL 240
LETVRFPLME AEVLQRLHDK LDPSPLRDTV ASALMYHRNE SLQPSLQSPQ TELRSDFQCV 300
VGFGGIHSTP STVLSDQAKY LNPLLGEWKH FTASLAPRMS NQGIAVLNNF VYLIGGDNNV 360
QGFRAESRCW RYDPRHNRWF QIQSLQQEHA DLSVCVVGRY IYAVAGRDYH NDLNAVERYD 420
PATNSWAYVA PLKREVYAHA GATLEGKMYI TCGRRGEDYL KETHCYDPGS NTWHTLADGP 480
VRRAWHGMAT LLNKLYVIGG SNNDAGYRRD VHQVACYSCT SGQWSSVCPL PAGHGEPGIA 540
VLDNRIYVLG GRSHNRGSRT GYVHIYDVEK DCWEEGPQLD NSISGLAACV LTLPRSLLLE 600
PPRGTPDRSQ ADPDFASEVM SVSDWEEFDN SSED 634 
Gene Ontology
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0031463; C:Cul3-RING ubiquitin ligase complex; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0072686; C:mitotic spindle; IDA:UniProtKB.
 GO:0005827; C:polar microtubule; IDA:UniProtKB.
 GO:0051301; P:cell division; IMP:UniProtKB.
 GO:0000070; P:mitotic sister chromatid segregation; IMP:UniProtKB.
 GO:0007094; P:mitotic spindle assembly checkpoint; IMP:UniProtKB.
 GO:0006513; P:protein monoubiquitination; IDA:UniProtKB. 
Interpro
 IPR011705; BACK.
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR013069; BTB_POZ.
 IPR017096; Kelch-like_gigaxonin.
 IPR015915; Kelch-typ_b-propeller.
 IPR006652; Kelch_1. 
Pfam
 PF07707; BACK
 PF00651; BTB
 PF01344; Kelch_1 
SMART
 SM00875; BACK
 SM00225; BTB
 SM00612; Kelch 
PROSITE
 PS50097; BTB 
PRINTS