CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022899
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin thioesterase ZRANB1 
Protein Synonyms/Alias
 TRAF-binding domain-containing protein; hTrabid; Zinc finger Ran-binding domain-containing protein 1 
Gene Name
 ZRANB1 
Gene Synonyms/Alias
 TRABID 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
77SSARPRVKSSYSMENubiquitination[1, 2, 3]
227TKRDSEVKMDFQRIEubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'- linked diubiquitin. Also cleaves 'Lys-63'-linked chains, but with 40-fold less efficiency compared to 'Lys-29'-linked ones. Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration. May also modulate TNF-alpha signaling. 
Sequence Annotation
 REPEAT 260 290 ANK 1.
 REPEAT 313 340 ANK 2.
 DOMAIN 432 592 OTU.
 ZN_FING 3 33 RanBP2-type 1.
 ZN_FING 84 113 RanBP2-type 2.
 ZN_FING 149 178 RanBP2-type 3.
 REGION 392 641 TRAF-binding.
 ACT_SITE 443 443 Nucleophile.
 ACT_SITE 585 585 Proton acceptor (By similarity).  
Keyword
 3D-structure; ANK repeat; Complete proteome; Cytoplasm; Hydrolase; Metal-binding; Nucleus; Protease; Reference proteome; Repeat; Thiol protease; Ubl conjugation pathway; Wnt signaling pathway; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 708 AA 
Protein Sequence
MSERGIKWAC EYCTYENWPS AIKCTMCRAQ RPSGTIITED PFKSGSSDVG RDWDPSSTEG 60
GSSPLICPDS SARPRVKSSY SMENANKWSC HMCTYLNWPR AIRCTQCLSQ RRTRSPTESP 120
QSSGSGSRPV AFSVDPCEEY NDRNKLNTRT QHWTCSVCTY ENWAKAKRCV VCDHPRPNNI 180
EAIELAETEE ASSIINEQDR ARWRGSCSSG NSQRRSPPAT KRDSEVKMDF QRIELAGAVG 240
SKEELEVDFK KLKQIKNRMK KTDWLFLNAC VGVVEGDLAA IEAYKSSGGD IARQLTADEV 300
RLLNRPSAFD VGYTLVHLAI RFQRQDMLAI LLTEVSQQAA KCIPAMVCPE LTEQIRREIA 360
ASLHQRKGDF ACYFLTDLVT FTLPADIEDL PPTVQEKLFD EVLDRDVQKE LEEESPIINW 420
SLELATRLDS RLYALWNRTA GDCLLDSVLQ ATWGIYDKDS VLRKALHDSL HDCSHWFYTR 480
WKDWESWYSQ SFGLHFSLRE EQWQEDWAFI LSLASQPGAS LEQTHIFVLA HILRRPIIVY 540
GVKYYKSFRG ETLGYTRFQG VYLPLLWEQS FCWKSPIALG YTRGHFSALV AMENDGYGNR 600
GAGANLNTDD DVTITFLPLV DSERKLLHVH FLSAQELGNE EQQEKLLREW LDCCVTEGGV 660
LVAMQKSSRR RNHPLVTQMV EKWLDRYRQI RPCTSLSDGE EDEDDEDE 708 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:HGNC.
 GO:0070530; F:K63-linked polyubiquitin binding; IDA:BHF-UCL.
 GO:0004221; F:ubiquitin thiolesterase activity; IMP:UniProtKB.
 GO:0004843; F:ubiquitin-specific protease activity; IDA:BHF-UCL.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0016477; P:cell migration; IMP:UniProtKB.
 GO:0007010; P:cytoskeleton organization; IMP:UniProtKB.
 GO:0030177; P:positive regulation of Wnt receptor signaling pathway; IMP:UniProtKB.
 GO:0071947; P:protein deubiquitination involved in ubiquitin-dependent protein catabolic process; IMP:BHF-UCL.
 GO:0070536; P:protein K63-linked deubiquitination; IMP:UniProtKB.
 GO:0022604; P:regulation of cell morphogenesis; IMP:UniProtKB.
 GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW. 
Interpro
 IPR003323; OTU.
 IPR001876; Znf_RanBP2. 
Pfam
 PF02338; OTU
 PF00641; zf-RanBP 
SMART
 SM00547; ZnF_RBZ 
PROSITE
 PS50088; ANK_REPEAT
 PS50802; OTU
 PS01358; ZF_RANBP2_1
 PS50199; ZF_RANBP2_2 
PRINTS