UniProt Accession

 TROP_HUMAN; Q12816; Q96SX2; Q9NU89; Q9UPN8 

Genbank Protein ID

 AB029037; AK027489; AL049732; AL049732; CH471154; U04811 

Genbank Nucleotide ID

 BAA83066.3; BAB55149.1; CAB86651.2; CAM26850.1; EAW93200.1; AAA79334.2 

Protein Name

 Trophinin 

Protein Synonyms/Alias

 MAGE-D3 antigen 

Gene Name

 TRO 

Gene Synonyms/Alias

 KIAA1114; MAGED3 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
81	APIKTITKAAPAAPP	ubiquitination	[1]
99	ANEIATNKPKITWQA	acetylation	[2]
301	AIRPKKSKGKKAASR	ubiquitination	[1]
453	ERANKLVKYLLVKDQ	ubiquitination	[1, 3, 4]
458	LVKYLLVKDQTKIPI	ubiquitination	[1]
462	LLVKDQTKIPIKRSD	ubiquitination	[1]
495	RASYTLEKMFRVNLK	ubiquitination	[5, 6]
502	KMFRVNLKEIDKQSS	ubiquitination	[5]
578	SLFGEVRKLITDEFV	ubiquitination	[6]
586	LITDEFVKQKYLEYK	ubiquitination	[1, 5]
588	TDEFVKQKYLEYKRV	ubiquitination	[1, 5]
593	KQKYLEYKRVPNSRP	ubiquitination	[1, 5]
617	RSYHETSKMKVLKFA	ubiquitination	[5]
622	TSKMKVLKFACRVQK	ubiquitination	[1]
633	RVQKKDPKDWAVQYR	ubiquitination	[6]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 

Functional Description

 Could be involved with bystin and tastin in a cell adhesion molecule complex that mediates an initial attachment of the blastocyst to uterine epithelial cells at the time of the embryo implantation. Directly responsible for homophilic cell adhesion. 

Sequence Annotation

 DOMAIN 444 642 MAGE.
 REPEAT 751 760 1.
 REPEAT 769 778 2.
 REPEAT 779 786 3; approximate.
 REPEAT 787 796 4.
 REPEAT 805 814 5.
 REPEAT 823 833 6; approximate.
 REPEAT 841 850 7.
 REPEAT 859 870 8; approximate.
 REPEAT 879 890 9; approximate.
 REPEAT 901 910 10.
 REPEAT 911 920 11.
 REPEAT 921 930 12.
 REPEAT 931 940 13.
 REPEAT 941 950 14.
 REPEAT 951 960 15; approximate.
 REPEAT 961 970 16.
 REPEAT 971 980 17.
 REPEAT 981 990 18.
 REPEAT 991 1000 19.
 REPEAT 1001 1010 20.
 REPEAT 1011 1020 21.
 REPEAT 1021 1030 22; approximate.
 REPEAT 1031 1040 23.
 REPEAT 1041 1050 24.
 REPEAT 1051 1060 25.
 REPEAT 1061 1070 26.
 REPEAT 1071 1080 27; approximate.
 REPEAT 1081 1090 28; approximate.
 REPEAT 1091 1100 29.
 REPEAT 1101 1110 30.
 REPEAT 1111 1120 31.
 REPEAT 1121 1130 32.
 REPEAT 1131 1140 33.
 REPEAT 1141 1150 34.
 REPEAT 1151 1160 35.
 REPEAT 1161 1170 36.
 REPEAT 1171 1180 37; approximate.
 REPEAT 1181 1190 38; approximate.
 REPEAT 1191 1200 39.
 REPEAT 1201 1210 40.
 REPEAT 1211 1220 41.
 REPEAT 1221 1230 42; approximate.
 REPEAT 1231 1240 43; approximate.
 REPEAT 1241 1250 44.
 REPEAT 1251 1260 45.
 REPEAT 1261 1270 46; approximate.
 REPEAT 1271 1280 47.
 REPEAT 1281 1290 48.
 REPEAT 1291 1300 49.
 REPEAT 1301 1310 50; approximate.
 REPEAT 1311 1320 51.
 REPEAT 1321 1330 52; approximate.
 REPEAT 1331 1340 53; approximate.
 REPEAT 1341 1350 54; approximate.
 REPEAT 1351 1360 55; approximate.
 REPEAT 1361 1370 56; approximate.
 REPEAT 1371 1380 57; approximate.
 REPEAT 1381 1390 58; approximate.
 REPEAT 1391 1400 59; approximate.
 REPEAT 1401 1410 60; approximate.
 REPEAT 1411 1420 61; approximate.
 REPEAT 1421 1430 62; approximate.
 REGION 751 1430 62 X 10 AA approximate tandem repeats.  

Keyword

 Alternative splicing; Cell adhesion; Complete proteome; Polymorphism; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1431 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0007566; P:embryo implantation; TAS:ProtInc.
 GO:0007156; P:homophilic cell adhesion; TAS:ProtInc.
 GO:0030308; P:negative regulation of cell growth; IEA:Compara. 

Interpro

 IPR002190; MAGE. 

Pfam

 PF01454; MAGE 

SMART

  

PROSITE

 PS50838; MAGE 

PRINTS