UniProt Accession

 PRS6B_YEAST; P33298; D6VT28 

Genbank Protein ID

 X73570; U06229; U32274; BK006938 

Genbank Nucleotide ID

 CAA51972.1; AAA81916.1; AAB64836.1; DAA12238.1 

Protein Name

 26S protease regulatory subunit 6B homolog 

Protein Synonyms/Alias

 Protein YNT1; Tat-binding homolog 2 

Gene Name

 RPT3 

Gene Synonyms/Alias

 YNT1; YTA2; YDR394W; D9509.14 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
224	TGKTMLVKAVANSTK	ubiquitination	[1]
231	KAVANSTKAAFIRVN	ubiquitination	[1]
245	NGSEFVHKYLGEGPR	acetylation	[2]
245	NGSEFVHKYLGEGPR	ubiquitination	[1]
280	EVDSIATKRFDAQTG	ubiquitination	[3]
337	RPGRLDRKIEFPSLR	acetylation	[2]
337	RPGRLDRKIEFPSLR	ubiquitination	[1]
416	EAYATQVKTDNTVDK	ubiquitination	[1]
423	KTDNTVDKFDFYK**	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

 The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity). 

Sequence Annotation

 NP_BIND 213 220 ATP (Potential).
 MOD_RES 1 1 N-acetylmethionine.  

Keyword

 3D-structure; Acetylation; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleotide-binding; Nucleus; Proteasome; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 428 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0008540; C:proteasome regulatory particle, base subcomplex; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016887; F:ATPase activity; ISS:SGD.
 GO:0045899; P:positive regulation of RNA polymerase II transcriptional preinitiation complex assembly; IMP:SGD.
 GO:0070682; P:proteasome regulatory particle assembly; IMP:SGD.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IMP:SGD. 

Interpro

 IPR005937; 26S_Psome_P45.
 IPR003593; AAA+_ATPase.
 IPR003959; ATPase_AAA_core.
 IPR003960; ATPase_AAA_CS.
 IPR027417; P-loop_NTPase. 

Pfam

 PF00004; AAA 

SMART

 SM00382; AAA 

PROSITE

 PS00674; AAA 

PRINTS