UniProt Accession

 ATIF1_RAT; Q03344; A0JMZ8 

Genbank Protein ID

 L07806; D13122; U12250; BC126065 

Genbank Nucleotide ID

 AAA41360.1; BAA02424.1; AAA85094.1; AAI26066.1 

Protein Name

 ATPase inhibitor, mitochondrial 

Protein Synonyms/Alias

 Inhibitor of F(1)F(o)-ATPase; IF(1); IF1 

Gene Name

 Atpif1 

Gene Synonyms/Alias

 Atpi; If1 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
49	EAGGAFGKREKAEED	acetylation	[1]
72	EQLAALKKHHEDEID	acetylation	[1]
83	DEIDHHSKEIERLQK	acetylation	[1]
90	KEIERLQKQIERHKK	acetylation	[1]
103	KKKIKYLKNSEH***	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Required to avoid the consumption of cellular ATP when the F(1)F(o)-ATP synthase enzyme acts as an ATP hydrolase. Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme (By similarity). 

Sequence Annotation

 REGION 26 52 N-terminal inhibitory region (By
 REGION 74 106 Antiparallel alpha-helical coiled coil  

Keyword

 Coiled coil; Complete proteome; Mitochondrion; Reference proteome; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 107 AA 

Protein Sequence

Gene Ontology

 GO:0009986; C:cell surface; ISS:UniProtKB.
 GO:0005739; C:mitochondrion; ISS:UniProtKB.
 GO:0043532; F:angiostatin binding; ISS:UniProtKB.
 GO:0051117; F:ATPase binding; ISS:UniProtKB.
 GO:0042030; F:ATPase inhibitor activity; ISS:UniProtKB.
 GO:0005516; F:calmodulin binding; ISS:UniProtKB.
 GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
 GO:0030218; P:erythrocyte differentiation; ISS:UniProtKB.
 GO:0006783; P:heme biosynthetic process; ISS:UniProtKB.
 GO:0001937; P:negative regulation of endothelial cell proliferation; ISS:UniProtKB.
 GO:0051346; P:negative regulation of hydrolase activity; ISS:UniProtKB.
 GO:0045980; P:negative regulation of nucleotide metabolic process; IEA:InterPro.
 GO:0051289; P:protein homotetramerization; ISS:UniProtKB. 

Interpro

 IPR007648; ATPase_inhibitor_IATP_mt. 

Pfam

 PF04568; IATP 

SMART

  

PROSITE

  

PRINTS