CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000024
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 AP-3 complex subunit beta-1 
Protein Synonyms/Alias
 Adapter-related protein complex 3 subunit beta-1; Adaptor protein complex AP-3 subunit beta-1; Beta-3A-adaptin; Clathrin assembly protein complex 3 beta-1 large chain 
Gene Name
 AP3B1 
Gene Synonyms/Alias
 ADTB3A 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
125STFQRALKDPNQLIRubiquitination[1]
168DLSPYVRKNAAHAIQubiquitination[2]
512ENCERVPKIAPDVLRubiquitination[3, 4]
523DVLRKMAKSFTSEDDubiquitination[3, 4]
552LTNSKQTKLLTQYILubiquitination[3, 4]
592VKSGALSKYAKKIFLacetylation[5]
596ALSKYAKKIFLAQKPubiquitination[3, 4]
602KKIFLAQKPAPLLESubiquitination[2, 3, 4]
658VEVIELAKEWTPAGKubiquitination[1, 2, 3, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. 
Sequence Annotation
 MOD_RES 276 276 Phosphoserine.
 MOD_RES 609 609 Phosphoserine.
 MOD_RES 750 750 Phosphoserine.
 MOD_RES 752 752 Phosphoserine.  
Keyword
 Albinism; Alternative splicing; Complete proteome; Cytoplasmic vesicle; Disease mutation; Endocytosis; Golgi apparatus; Hermansky-Pudlak syndrome; Membrane; Phosphoprotein; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1094 AA 
Protein Sequence
MSSNSFPYNE QSGGGEATEL GQEATSTISP SGAFGLFSSD LKKNEDLKQM LESNKDSAKL 60
DAMKRIVGMI AKGKNASELF PAVVKNVASK NIEIKKLVYV YLVRYAEEQQ DLALLSISTF 120
QRALKDPNQL IRASALRVLS SIRVPIIVPI MMLAIKEASA DLSPYVRKNA AHAIQKLYSL 180
DPEQKEMLIE VIEKLLKDKS TLVAGSVVMA FEEVCPDRID LIHKNYRKLC NLLVDVEEWG 240
QVVIIHMLTR YARTQFVSPW KEGDELEDNG KNFYESDDDQ KEKTDKKKKP YTMDPDHRLL 300
IRNTKPLLQS RNAAVVMAVA QLYWHISPKS EAGIISKSLV RLLRSNREVQ YIVLQNIATM 360
SIQRKGMFEP YLKSFYVRST DPTMIKTLKL EILTNLANEA NISTLLREFQ TYVKSQDKQF 420
AAATIQTIGR CATNILEVTD TCLNGLVCLL SNRDEIVVAE SVVVIKKLLQ MQPAQHGEII 480
KHMAKLLDSI TVPVARASIL WLIGENCERV PKIAPDVLRK MAKSFTSEDD LVKLQILNLG 540
AKLYLTNSKQ TKLLTQYILN LGKYDQNYDI RDRTRFIRQL IVPNVKSGAL SKYAKKIFLA 600
QKPAPLLESP FKDRDHFQLG TLSHTLNIKA TGYLELSNWP EVAPDPSVRN VEVIELAKEW 660
TPAGKAKQEN SAKKFYSESE EEEDSSDSSS DSESESGSES GEQGESGEEG DSNEDSSEDS 720
SSEQDSESGR ESGLENKRTA KRNSKAKGKS DSEDGEKENE KSKTSDSSND ESSSIEDSSS 780
DSESESEPES ESESRRVTKE KEKKTKQDRT PLTKDVSLLD LDDFNPVSTP VALPTPALSP 840
SLMADLEGLH LSTSSSVISV STPAFVPTKT HVLLHRMSGK GLAAHYFFPR QPCIFGDKMV 900
SIQITLNNTT DRKIENIHIG EKKLPIGMKM HVFNPIDSLE PEGSITVSMG IDFCDSTQTA 960
SFQLCTKDDC FNVNIQPPVG ELLLPVAMSE KDFKKEQGVL TGMNETSAVI IAAPQNFTPS 1020
VIFQKVVNVA NVGAVPSGQD NIHRFAAKTV HSGSLMLVTV ELKEGSTAQL IINTEKTVIG 1080
SVLLRELKPV LSQG 1094 
Gene Ontology
 GO:0030123; C:AP-3 adaptor complex; IEA:InterPro.
 GO:0030665; C:clathrin-coated vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0005794; C:Golgi apparatus; TAS:ProtInc.
 GO:0008089; P:anterograde axon cargo transport; ISS:UniProtKB.
 GO:0048490; P:anterograde synaptic vesicle transport; ISS:UniProtKB.
 GO:0048007; P:antigen processing and presentation, exogenous lipid antigen via MHC class Ib; IEA:Compara.
 GO:0007596; P:blood coagulation; IEA:Compara.
 GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
 GO:0006886; P:intracellular protein transport; TAS:ProtInc.
 GO:0032438; P:melanosome organization; IMP:UniProtKB.
 GO:0051138; P:positive regulation of NK T cell differentiation; IEA:Compara.
 GO:0006622; P:protein targeting to lysosome; IEA:Compara. 
Interpro
 IPR026740; AP3_beta.
 IPR026739; AP_beta.
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR002553; Clathrin/coatomer_adapt-like_N. 
Pfam
 PF01602; Adaptin_N 
SMART
  
PROSITE
  
PRINTS