UniProt Accession

 MKNK2_HUMAN; Q9HBH9; Q6GPI3; Q9HBH8; Q9UHR0; Q9Y2N6 

Genbank Protein ID

 AF237775; AF237776; AF125532; BC073140 

Genbank Nucleotide ID

 AAG26336.1; AAG26337.1; AAF17226.1; AAH73140.1 

Protein Name

 MAP kinase-interacting serine/threonine-protein kinase 2 

Protein Synonyms/Alias

 MAP kinase signal-integrating kinase 2; MAPK signal-integrating kinase 2; Mnk2 

Gene Name

 MKNK2 

Gene Synonyms/Alias

 GPRK7; MNK2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
173	SILSHIHKRRHFNEL	ubiquitination	[1]
326	FESIQEGKYEFPDKD	ubiquitination	[2]
347	AAKDLISKLLVRDAK	ubiquitination	[1]
390	LQRNSCAKDLTSFAA	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Serine/threonine-protein kinase that phosphorylates SFPQ/PSF, HNRNPA1 and EIF4E. May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap. Required for mediating PP2A-inhibition-induced EIF4E phosphorylation. Triggers EIF4E shuttling from cytoplasm to nucleus. Isoform 1 displays a high basal kinase activity, but isoform 2 exhibits a very low kinase activity. Acts as a mediator of the suppressive effects of IFNgamma on hematopoiesis. Negative regulator for signals that control generation of arsenic trioxide As(2)O(3)-dependent apoptosis and anti-leukemic responses. Involved in anti-apoptotic signaling in response to serum withdrawal. 

Sequence Annotation

 DOMAIN 84 388 Protein kinase.
 NP_BIND 90 98 ATP (By similarity).
 REGION 160 162 Staurosporine binding.
 MOTIF 60 66 Nuclear localization signal.
 MOTIF 444 448 MAP kinase binding (By similarity).
 ACT_SITE 205 205 Proton acceptor (By similarity).
 METAL 299 299 Zinc.
 METAL 311 311 Zinc.
 METAL 314 314 Zinc.
 BINDING 113 113 ATP (By similarity).
 BINDING 209 209 Staurosporine.
 MOD_RES 74 74 Phosphoserine (By similarity).
 MOD_RES 244 244 Phosphothreonine.
 MOD_RES 249 249 Phosphothreonine.
 MOD_RES 379 379 Phosphothreonine.
 MOD_RES 437 437 Phosphoserine (By similarity).
 MOD_RES 440 440 Phosphoserine (By similarity).  

Keyword

 3D-structure; Alternative splicing; Apoptosis; ATP-binding; Complete proteome; Cytoplasm; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase; Translation regulation; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 465 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016605; C:PML body; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0007166; P:cell surface receptor signaling pathway; TAS:ProtInc.
 GO:0071243; P:cellular response to arsenic-containing substance; IDA:UniProtKB.
 GO:0030097; P:hemopoiesis; IDA:UniProtKB.
 GO:0007243; P:intracellular protein kinase cascade; IDA:UniProtKB.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF00069; Pkinase 

SMART

 SM00220; S_TKc 

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS