UniProt Accession

 ACAP1_HUMAN; Q15027; Q53XN9 

Genbank Protein ID

 D30758; BT009788; BC018543 

Genbank Nucleotide ID

 BAA06418.2; AAP88790.1; AAH18543.1 

Protein Name

 Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 

Protein Synonyms/Alias

 Centaurin-beta-1; Cnt-b1 

Gene Name

 ACAP1 

Gene Synonyms/Alias

 CENTB1; KIAA0050 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
4	****MTVKLDFEECL	ubiquitination	[1]
12	LDFEECLKDSPRFRA	ubiquitination	[1, 2]
40	TRLEKLLKLGTGLLE	ubiquitination	[1, 2]
82	MMAECLEKFTVSLNH	ubiquitination	[1]
90	FTVSLNHKLDSHAEL	ubiquitination	[1]
114	QQIQTLVKEGLRGFR	ubiquitination	[1]
183	QINVIEDKRKFDIME	ubiquitination	[1]
185	NVIEDKRKFDIMEFV	ubiquitination	[1]
218	SRLSQYRKELGAQLH	ubiquitination	[1, 2]
248	RHVLLKQKELGGEEP	ubiquitination	[1, 2, 3]
274	VMEGHLFKRASNAFK	acetylation	[2, 4]
274	VMEGHLFKRASNAFK	ubiquitination	[1, 2]
281	KRASNAFKTWSRRWF	ubiquitination	[1]
300	NQLVYQKKYKDPVTV	ubiquitination	[1]
302	LVYQKKYKDPVTVVV	ubiquitination	[1, 2]
318	DLRLCTVKLCPDSER	ubiquitination	[1, 2]
456	SLGVHFSKVRSLTLD	ubiquitination	[1]
495	RVEAMAVKKPGPSCS	ubiquitination	[1, 2]
506	PSCSRQEKEAWIHAK	ubiquitination	[1]
513	KEAWIHAKYVEKKFL	ubiquitination	[1]
522	VEKKFLTKLPEIRGR	ubiquitination	[1, 2]
558	RPGSLRSKPEPPSED	ubiquitination	[1, 2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 

Functional Description

 GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. 

Sequence Annotation

 DOMAIN 1 226 BAR.
 DOMAIN 265 360 PH.
 DOMAIN 405 527 Arf-GAP.
 REPEAT 606 635 ANK 1.
 REPEAT 639 668 ANK 2.
 REPEAT 672 702 ANK 3.
 ZN_FING 420 443 C4-type.
 REGION 1 382 Required for formation of endosomal
 REGION 405 740 Required for interaction with GULP1.
 MOD_RES 485 485 Nitrated tyrosine.
 MOD_RES 554 554 Phosphoserine; by PKB.  

Keyword

 3D-structure; ANK repeat; Complete proteome; GTPase activation; Metal-binding; Nitration; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Repeat; Transport; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 740 AA 

Protein Sequence

Gene Ontology

 GO:0008060; F:ARF GTPase activator activity; IEA:InterPro.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0043547; P:positive regulation of GTPase activity; IEA:GOC.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0032312; P:regulation of ARF GTPase activity; IEA:InterPro. 

Interpro

 IPR027267; AH/BAR-dom.
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR001164; ArfGAP.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR000909; PLipase_C_PInositol-sp_X_dom. 

Pfam

 PF00023; Ank
 PF01412; ArfGap
 PF00169; PH 

SMART

 SM00248; ANK
 SM00105; ArfGap
 SM00233; PH 

PROSITE

 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT
 PS50115; ARFGAP
 PS51021; BAR
 PS50003; PH_DOMAIN 

PRINTS

 PR00405; REVINTRACTNG.