CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018747
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Hamartin 
Protein Synonyms/Alias
 Tuberous sclerosis 1 protein 
Gene Name
 TSC1 
Gene Synonyms/Alias
 KIAA0243; TSC 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
238PELVTGSKDHELDPRubiquitination[1]
477EDSIEKDKEEAAISRubiquitination[2]
672NKLPLPSKSVDWTHFubiquitination[1]
725RLLRKVIKAAALEEHubiquitination[2]
756MWKVSLQKEQARYNQubiquitination[1]
774QRDTMVTKLHSQIRQubiquitination[1]
928HLLLEQKKYLEDVKLubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Seems not to be required for TSC2 GAP activity towards RHEB. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. 
Sequence Annotation
 MOD_RES 394 394 Phosphoserine (By similarity).
 MOD_RES 505 505 Phosphoserine.
 MOD_RES 511 511 Phosphoserine.
 MOD_RES 598 598 Phosphoserine.  
Keyword
 Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Disease mutation; Epilepsy; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Tumor suppressor. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1164 AA 
Protein Sequence
MAQQANVGEL LAMLDSPMLG VRDDVTAVFK ENLNSDRGPM LVNTLVDYYL ETSSQPALHI 60
LTTLQEPHDK HLLDRINEYV GKAATRLSIL SLLGHVIRLQ PSWKHKLSQA PLLPSLLKCL 120
KMDTDVVVLT TGVLVLITML PMIPQSGKQH LLDFFDIFGR LSSWCLKKPG HVAEVYLVHL 180
HASVYALFHR LYGMYPCNFV SFLRSHYSMK ENLETFEEVV KPMMEHVRIH PELVTGSKDH 240
ELDPRRWKRL ETHDVVIECA KISLDPTEAS YEDGYSVSHQ ISARFPHRSA DVTTSPYADT 300
QNSYGCATST PYSTSRLMLL NMPGQLPQTL SSPSTRLITE PPQATLWSPS MVCGMTTPPT 360
SPGNVPPDLS HPYSKVFGTT AGGKGTPLGT PATSPPPAPL CHSDDYVHIS LPQATVTPPR 420
KEERMDSARP CLHRQHHLLN DRGSEEPPGS KGSVTLSDLP GFLGDLASEE DSIEKDKEEA 480
AISRELSEIT TAEAEPVVPR GGFDSPFYRD SLPGSQRKTH SAASSSQGAS VNPEPLHSSL 540
DKLGPDTPKQ AFTPIDLPCG SADESPAGDR ECQTSLETSI FTPSPCKIPP PTRVGFGSGQ 600
PPPYDHLFEV ALPKTAHHFV IRKTEELLKK AKGNTEEDGV PSTSPMEVLD RLIQQGADAH 660
SKELNKLPLP SKSVDWTHFG GSPPSDEIRT LRDQLLLLHN QLLYERFKRQ QHALRNRRLL 720
RKVIKAAALE EHNAAMKDQL KLQEKDIQMW KVSLQKEQAR YNQLQEQRDT MVTKLHSQIR 780
QLQHDREEFY NQSQELQTKL EDCRNMIAEL RIELKKANNK VCHTELLLSQ VSQKLSNSES 840
VQQQMEFLNR QLLVLGEVNE LYLEQLQNKH SDTTKEVEMM KAAYRKELEK NRSHVLQQTQ 900
RLDTSQKRIL ELESHLAKKD HLLLEQKKYL EDVKLQARGQ LQAAESRYEA QKRITQVFEL 960
EILDLYGRLE KDGLLKKLEE EKAEAAEAAE ERLDCCNDGC SDSMVGHNEE ASGHNGETKT 1020
PRPSSARGSS GSRGGGGSSS SSSELSTPEK PPHQRAGPFS SRWETTMGEA SASIPTTVGS 1080
LPSSKSFLGM KARELFRNKS ESQCDEDGMT SSLSESLKTE LGKDLGVEAK IPLNLDGPHP 1140
SPPTPDSVGQ LHIMDYNETH HEHS 1164 
Gene Ontology
 GO:0005938; C:cell cortex; IDA:UniProtKB.
 GO:0030426; C:growth cone; IEA:Compara.
 GO:0043231; C:intracellular membrane-bounded organelle; IEA:Compara.
 GO:0030027; C:lamellipodium; IDA:UniProtKB.
 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0033596; C:TSC1-TSC2 complex; IDA:UniProtKB.
 GO:0030695; F:GTPase regulator activity; IEA:Compara.
 GO:0032862; P:activation of Rho GTPase activity; IDA:UniProtKB.
 GO:0055007; P:cardiac muscle cell differentiation; IEA:Compara.
 GO:0007050; P:cell cycle arrest; TAS:Reactome.
 GO:0030030; P:cell projection organization; IEA:Compara.
 GO:0007160; P:cell-matrix adhesion; IMP:UniProtKB.
 GO:0021987; P:cerebral cortex development; IEA:Compara.
 GO:0021766; P:hippocampus development; IEA:Compara.
 GO:0008286; P:insulin receptor signaling pathway; TAS:Reactome.
 GO:0001822; P:kidney development; IEA:Compara.
 GO:0042552; P:myelination; IEA:Compara.
 GO:0008285; P:negative regulation of cell proliferation; IMP:UniProtKB.
 GO:0045792; P:negative regulation of cell size; IEA:Compara.
 GO:0032007; P:negative regulation of TOR signaling cascade; IMP:UniProtKB.
 GO:0017148; P:negative regulation of translation; IMP:UniProtKB.
 GO:0001843; P:neural tube closure; IEA:Compara.
 GO:0051894; P:positive regulation of focal adhesion assembly; IDA:UniProtKB.
 GO:0006813; P:potassium ion transport; IEA:Compara.
 GO:0051291; P:protein heterooligomerization; IEA:Compara.
 GO:0050821; P:protein stabilization; IDA:UniProtKB.
 GO:0043666; P:regulation of phosphoprotein phosphatase activity; IMP:UniProtKB.
 GO:0045859; P:regulation of protein kinase activity; IEA:Compara.
 GO:0051492; P:regulation of stress fiber assembly; IDA:UniProtKB.
 GO:0006407; P:rRNA export from nucleus; IMP:UniProtKB.
 GO:0050808; P:synapse organization; IEA:Compara. 
Interpro
 IPR007483; Hamartin. 
Pfam
 PF04388; Hamartin 
SMART
  
PROSITE
  
PRINTS