CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015917
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin-protein ligase E3B 
Protein Synonyms/Alias
  
Gene Name
 UBE3B 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
436LLKRAFQKSASVRNIubiquitination[1]
751DPALNLFKTTSGDERubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (By similarity). 
Sequence Annotation
 DOMAIN 29 58 IQ.
 DOMAIN 702 1068 HECT.
 ACT_SITE 1036 1036 Glycyl thioester intermediate (By
 MOD_RES 419 419 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Ligase; Phosphoprotein; Polymorphism; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1068 AA 
Protein Sequence
MFTLSQTSRA WFIDRARQAR EERLVQKERE RAAVVIQAHV RSFLCRSRLQ RDIRREIDDF 60
FKADDPESTK RSALCIFKIA RKLLFLFRIK EDNERFEKLC RSILSSMDAE NEPKVWYVSL 120
ACSKDLTLLW IQQIKNILWY CCDFLKQLKP EILQDSRLIT LYLTMLVTFT DTSTWKILRG 180
KGESLRPAMN HICANIMGHL NQHGFYSVLQ ILLTRGLARP RPCLSKGTLT AAFSLALRPV 240
IAAQFSDNLI RPFLIHIMSV PALVTHLSTV TPERLTVLES HDMLRKFIIF LRDQDRCRDV 300
CESLEGCHTL CLMGNLLHLG SLSPRVLEEE TDGFVSLLTQ TLCYCRKYVS QKKSNLTHWH 360
PVLGWFSQSV DYGLNESMHL ITKQLQFLWG VPLIRIFFCD ILSKKLLESQ EPAHAQPASP 420
QNVLPVKSLL KRAFQKSASV RNILRPVGGK RVDSAEVQKV CNICVLYQTS LTTLTQIRLQ 480
ILTGLTYLDD LLPKLWAFIC ELGPHGGLKL FLECLNNDTE ESKQLLAMLM LFCDCSRHLI 540
TILDDIEVYE EQISFKLEEL VTISSFLNSF VFKMIWDGIV ENAKGETLEL FQSVHGWLMV 600
LYERDCRRRF TPEDHWLRKD LKPSVLFQEL DRDRKRAQLI LQYIPHVIPH KNRVLLFRTM 660
VTKEKEKLGL VETSSASPHV THITIRRSRM LEDGYEQLRQ LSQHAMKGVI RVKFVNDLGV 720
DEAGIDQDGV FKEFLEEIIK RVFDPALNLF KTTSGDERLY PSPTSYIHEN YLQLFEFVGK 780
MLGKAVYEGI VVDVPFASFF LSQLLGHHHS VFYSSVDELP SLDSEFYKNL TSIKRYDGDI 840
TDLGLTLSYD EDVMGQLVCH ELIPGGKTIP VTNENKISYI HLMAHFRMHT QIKNQTAALI 900
SGFRSIIKPE WIRMFSTPEL QRLISGDNAE IDLEDLKKHT VYYGGFHGSH RVIIWLWDIL 960
ASDFTPDERA MFLKFVTSCS RPPLLGFAYL KPPFSIRCVE VSDDQDTGDT LGSVLRGFFT 1020
IRKREPGGRL PTSSTCFNLL KLPNYSKKSV LREKLRYAIS MNTGFELS 1068 
Gene Ontology
 GO:0005737; C:cytoplasm; IBA:RefGenome.
 GO:0005634; C:nucleus; IBA:RefGenome.
 GO:0004842; F:ubiquitin-protein ligase activity; IBA:RefGenome.
 GO:0042787; P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process; IBA:RefGenome. 
Interpro
 IPR000569; HECT.
 IPR000048; IQ_motif_EF-hand-BS. 
Pfam
 PF00632; HECT
 PF00612; IQ 
SMART
 SM00119; HECTc
 SM00015; IQ 
PROSITE
 PS50237; HECT
 PS50096; IQ 
PRINTS