CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006366
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sterol esterase TGL1 
Protein Synonyms/Alias
 Triglyceride lipase-cholesterol esterase 1 
Gene Name
 TGL1 
Gene Synonyms/Alias
 YKL140W; YKL5 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
100HRIPPISKNRFNNKVubiquitination[1]
246RIVDTLAKSSPGFMYubiquitination[2]
259MYLFFGRKIVLPSAVubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Mediates the hydrolysis of steryl esters. Required for mobilization of steryl ester, thereby playing a central role in lipid metabolism. May have weak lipase activity toward triglycerides upon some conditions, however, the relevance of such activity is unclear in vivo. 
Sequence Annotation
 ACT_SITE 201 201 Nucleophile (By similarity).
 ACT_SITE 369 369 Charge relay system (By similarity).
 ACT_SITE 396 396 Charge relay system (By similarity).
 MOD_RES 462 462 Phosphoserine.
 MOD_RES 466 466 Phosphoserine.
 MOD_RES 538 538 Phosphoserine.
 MOD_RES 539 539 Phosphothreonine.  
Keyword
 Complete proteome; Hydrolase; Lipid degradation; Lipid droplet; Lipid metabolism; Membrane; Phosphoprotein; Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 548 AA 
Protein Sequence
MYFPFLGRLS ITDYIIVVLV YIESIISSVL KLIPQPMINL FEWLINFSTS SDDNTIEEKL 60
RSAPTIHEMC AIFDISVEDH LVRTEDNYIL TLHRIPPISK NRFNNKVVYL HHGLLMCSDV 120
WCCNIERHKN LPFVLHDLGY DVWMGNNRGN KYSTAHLNKP PKSNKFWDFS IDEFAFFDIP 180
NSIEFILDIT KVDKVICIGF SQGSAQMFAA FSLSEKLNRK VSHFIAIAPA MTPKGLHNRI 240
VDTLAKSSPG FMYLFFGRKI VLPSAVIWQR TLHPTLFNLC IDIANKILFN WKSFNILPRQ 300
KIASYAKLYS TTSVKSIVHW FQILRSQKFQ MFEESDNMLN SLTRPYQIAN FPTRTNIKIP 360
ILLIYGGIDS LVDIDVMKKN LPFNSVFDVK VDNYEHLDLI WGKDADTLVI AKVLRFIEFF 420
NPGNVSVKTN QLLPSASLVE ELPSTTWKTT HPTHGLSYRT HSADRSPLSV QADEADEVHN 480
ADNSRFLRRV FSTSAIDEDN ENEHQDDTED QIHKEQQRRL SAYLESSKDL RQLDANSSTT 540
ALDALNKE 548 
Gene Ontology
 GO:0016021; C:integral to membrane; IDA:SGD.
 GO:0005811; C:lipid particle; IDA:SGD.
 GO:0016298; F:lipase activity; ISS:SGD.
 GO:0004771; F:sterol esterase activity; IDA:SGD.
 GO:0044255; P:cellular lipid metabolic process; IMP:SGD.
 GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
 GO:0016125; P:sterol metabolic process; IMP:SGD. 
Interpro
 IPR000073; AB_hydrolase_1.
 IPR006693; AB_hydrolase_lipase. 
Pfam
 PF04083; Abhydro_lipase
 PF00561; Abhydrolase_1 
SMART
  
PROSITE
 PS00120; LIPASE_SER 
PRINTS