UniProt Accession

 CELF2_RAT; Q792H5; A1L1J5; O88756; Q78ZF0 

Genbank Protein ID

 AF090695; AJ010351; AJ010386; BC129096 

Genbank Nucleotide ID

 AAD13762.1; CAA09102.1; CAA09103.1; AAI29097.1 

Protein Name

 CUGBP Elav-like family member 2 

Protein Synonyms/Alias

 CELF-2; Bruno-like protein 3; CUG triplet repeat RNA-binding protein 2; CUG-BP2; CUG-BP- and ETR-3-like factor 2; ELAV-type RNA-binding protein 3; ETR-3; Protein ETR-R3; Neuroblastoma apoptosis-related RNA-binding protein; rNapor; RNA-binding protein BRUNOL-3 

Gene Name

 Celf2 

Gene Synonyms/Alias

 Cugbp2; Etr3; Napor 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
94	FVTFYTRKAALEAQN	ubiquitination	[1]
458	SAKVFIDKQTNLSKC	ubiquitination	[1]

Reference

 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

 RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of TNNT2 in embryonic, but not adult, skeletal muscle. Activates TNNT2 exon 5 inclusion by antagonizing the repressive effect of PTB. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Promotes inclusion of exonS 21 and exclusion of exon 5 of the NMDA receptor R1 pre- mRNA. Involved in the apoB RNA editing activity. Increases COX2 mRNA stability and inhibits COX2 mRNA translation in epithelial cells after radiation injury. Modulates the cellular apoptosis program by regulating COX2-mediated prostaglandin E2 (PGE2) expression. Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK. Binds to the muscle-specific splicing enhancer (MSE) intronic sites flanking the TNNT2 alternative exon 5. Binds preferentially to UG-rich sequences, in particular UG repeat and UGUU motifs. Binds to apoB mRNA, specifically to AU- rich sequences located immediatly upstream of the edited cytidine. Binds AU-rich sequences in the 3'-UTR of COX2 mRNA. Binds to an intronic RNA element responsible for the silencing of exon 21 splicing. Binds to (CUG)n repeats (By similarity). 

Sequence Annotation

 DOMAIN 40 123 RRM 1.
 DOMAIN 132 212 RRM 2.
 DOMAIN 423 501 RRM 3.
 REGION 1 283 Necessary for RNA-binding, TNNT2 exon 5
 REGION 357 508 Necessary for RNA-binding, TNNT2 exon 5  

Keyword

 Alternative splicing; Complete proteome; Cytoplasm; mRNA processing; Nucleus; Reference proteome; Repeat; Repressor; RNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 508 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IDA:RGD.
 GO:0006376; P:mRNA splice site selection; IEA:Compara. 

Interpro

 IPR002343; Hud_Sxl_RNA.
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 

Pfam

 PF00076; RRM_1 

SMART

 SM00360; RRM 

PROSITE

 PS50102; RRM 

PRINTS

 PR00961; HUDSXLRNA.