CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014569
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 KN motif and ankyrin repeat domain-containing protein 2 
Protein Synonyms/Alias
 Ankyrin repeat domain-containing protein 25; Matrix-remodeling-associated protein 3; SRC-1-interacting protein; SIP; SRC-interacting protein; SRC1-interacting protein 
Gene Name
 KANK2 
Gene Synonyms/Alias
 ANKRD25; KIAA1518; MXRA3; SIP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
223LSVLQEEKRQLTVQLubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
 Involved in transcription regulation by sequestering nuclear receptor coactivators, such as NCOA1, NCOA2 and NCOA3, in the cytoplasm; the function is deregulated by phosphorylation. May be involved in the control of cytoskeleton formation by regulating actin polymerization. Involved in regulation of caspase- independent apoptosis; proposed to sequester AIFM1 in mitochondria and apoptotic stimuli lead to its proteasomal degradation allowing the release of AIFM1 to the nucleus. May be involved in promotion of cell proliferation. 
Sequence Annotation
 REPEAT 666 696 ANK 1.
 REPEAT 700 733 ANK 2.
 REPEAT 738 767 ANK 3.
 REPEAT 771 801 ANK 4.
 REPEAT 805 835 ANK 5.
 REGION 1 72 Interaction with AIFM1.
 REGION 669 835 Interaction with NCOA1.
 MOD_RES 19 19 Phosphoserine.
 MOD_RES 246 246 Phosphoserine (By similarity).
 MOD_RES 356 356 Phosphoserine.
 MOD_RES 375 375 Phosphoserine.
 MOD_RES 472 472 Phosphothreonine.  
Keyword
 3D-structure; Alternative splicing; ANK repeat; Apoptosis; Coiled coil; Complete proteome; Cytoplasm; Mitochondrion; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 851 AA 
Protein Sequence
MAQVLHVPAP FPGTPGPASP PAFPAKDPDP PYSVETPYGY RLDLDFLKYV DDIEKGHTLR 60
RVAVQRRPRL SSLPRGPGSW WTSTESLCSN ASGDSRHSAY SYCGRGFYPQ YGALETRGGF 120
NPRVERTLLD ARRRLEDQAA TPTGLGSLTP SAAGSTASLV GVGLPPPTPR SSGLSTPVPP 180
SAGHLAHVRE QMAGALRKLR QLEEQVKLIP VLQVKLSVLQ EEKRQLTVQL KSQKFLGHPT 240
AGRGRSELCL DLPDPPEDPV ALETRSVGTW VRERDLGMPD GEAALAAKVA VLETQLKKAL 300
QELQAAQARQ ADPQPQAWPP PDSPVRVDTV RVVEGPREVE VVASTAAGAP AQRAQSLEPY 360
GTGLRALAMP GRPESPPVFR SQEVVETMCP VPAAATSNVH MVKKISITER SCDGAAGLPE 420
VPAESSSSPP GSEVASLTQP EKSTGRVPTQ EPTHREPTRQ AASQESEEAG GTAPPLSSPP 480
GGPPAGVRSI MKRKEEVADP TAHRRSLQFV GVNGGYESSS EDSSTAENIS DNDSTENEAP 540
EPRERVPSVA EAPQLRPAGT AAAKTSRQEC QLSRESQHIP TAEGASGSNT EEEIRMELSP 600
DLISACLALE KYLDNPNALT ERELKVAYTT VLQEWLRLAC RSDAHPELVR RHLVTFRAMS 660
ARLLDYVVNI ADSNGNTALH YSVSHANFPV VQQLLDSGVC KVDKQNRAGY SPIMLTALAT 720
LKTQDDIETV LQLFRLGNIN AKASQAGQTA LMLAVSHGRV DVVKALLACE ADVNVQDDDG 780
STALMCACEH GHKEIAGLLL AVPSCDISLT DRDGSTALMV ALDAGQSEIA SMLYSRMNIK 840
CSFAPMSDDE SPTSSSAEE 859 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:UniProtKB.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0008285; P:negative regulation of cell proliferation; IDA:UniProtKB.
 GO:2000134; P:negative regulation of G1/S transition of mitotic cell cycle; IDA:UniProtKB.
 GO:0033147; P:negative regulation of intracellular estrogen receptor signaling pathway; IDA:UniProtKB.
 GO:0043069; P:negative regulation of programmed cell death; IDA:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR021939; KN_motif. 
Pfam
 PF00023; Ank
 PF12796; Ank_2
 PF12075; KN_motif 
SMART
 SM00248; ANK 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT 
PRINTS