CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022843
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 7-dehydrocholesterol reductase 
Protein Synonyms/Alias
 7-DHC reductase; Putative sterol reductase SR-2; Sterol Delta(7)-reductase 
Gene Name
 DHCR7 
Gene Synonyms/Alias
 D7SR 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
4****MAAKSQPNIPKubiquitination[1, 2]
13QPNIPKAKSLDGVTNubiquitination[2, 3]
88RLSDIWAKTPPITRKubiquitination[1, 2, 3, 4, 5, 6]
231EFNPRIGKWFDFKLFubiquitination[3]
358FRVANHQKDLFRRTDubiquitination[3, 4, 6]
374RCLIWGRKPKVIECSubiquitination[2]
376LIWGRKPKVIECSYTubiquitination[2]
454DEHRCASKYGRDWERubiquitination[3]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC). 
Sequence Annotation
 MOD_RES 14 14 Phosphoserine.  
Keyword
 Cholesterol biosynthesis; Cholesterol metabolism; Complete proteome; Disease mutation; Endoplasmic reticulum; Lipid biosynthesis; Lipid metabolism; Membrane; NADP; Oxidoreductase; Phosphoprotein; Polymorphism; Reference proteome; Steroid biosynthesis; Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 475 AA 
Protein Sequence
MAAKSQPNIP KAKSLDGVTN DRTASQGQWG RAWEVDWFSL ASVIFLLLFA PFIVYYFIMA 60
CDQYSCALTG PVVDIVTGHA RLSDIWAKTP PITRKAAQLY TLWVTFQVLL YTSLPDFCHK 120
FLPGYVGGIQ EGAVTPAGVV NKYQINGLQA WLLTHLLWFA NAHLLSWFSP TIIFDNWIPL 180
LWCANILGYA VSTFAMVKGY FFPTSARDCK FTGNFFYNYM MGIEFNPRIG KWFDFKLFFN 240
GRPGIVAWTL INLSFAAKQR ELHSHVTNAM VLVNVLQAIY VIDFFWNETW YLKTIDICHD 300
HFGWYLGWGD CVWLPYLYTL QGLYLVYHPV QLSTPHAVGV LLLGLVGYYI FRVANHQKDL 360
FRRTDGRCLI WGRKPKVIEC SYTSADGQRH HSKLLVSGFW GVARHFNYVG DLMGSLAYCL 420
ACGGGHLLPY FYIIYMAILL THRCLRDEHR CASKYGRDWE RYTAAVPYRL LPGIF 475 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005640; C:nuclear outer membrane; IDA:UniProtKB.
 GO:0047598; F:7-dehydrocholesterol reductase activity; IDA:UniProtKB.
 GO:0001568; P:blood vessel development; IEA:Compara.
 GO:0030154; P:cell differentiation; IEA:Compara.
 GO:0006695; P:cholesterol biosynthetic process; IMP:UniProtKB.
 GO:0030324; P:lung development; IEA:Compara.
 GO:0035264; P:multicellular organism growth; IEA:Compara.
 GO:0009791; P:post-embryonic development; IEA:Compara.
 GO:0042127; P:regulation of cell proliferation; IEA:Compara.
 GO:0045540; P:regulation of cholesterol biosynthetic process; IEA:Compara. 
Interpro
 IPR001171; Ergosterol_biosynth_ERG4_ERG24.
 IPR018083; Sterol_reductase_CS. 
Pfam
 PF01222; ERG4_ERG24 
SMART
  
PROSITE
 PS01017; STEROL_REDUCT_1
 PS01018; STEROL_REDUCT_2 
PRINTS