CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020224
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 
Protein Synonyms/Alias
 Acireductone dioxygenase (Fe(2+)-requiring); ARD; Fe-ARD; Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1; MTCBP-1; Submergence-induced protein-like factor; Sip-L 
Gene Name
 ADI1 
Gene Synonyms/Alias
 MTCBP1; HMFT1638 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
40RLGVLYWKLDADKYEubiquitination[1, 2]
45YWKLDADKYENDPELubiquitination[1, 2, 3]
54ENDPELEKIRRERNYubiquitination[2]
73IITICKDKLPNYEEKacetylation[4]
73IITICKDKLPNYEEKubiquitination[2, 4]
80KLPNYEEKIKMFYEEubiquitination[1, 2]
110GYFDVRDKEDQWIRIubiquitination[2, 4]
121WIRIFMEKGDMVTLPubiquitination[2, 5]
140HRFTVDEKNYTKAMRubiquitination[4]
173EARGQYVKFLAQTA*ubiquitination[1, 2]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
 Catalyzes the formation of formate and 2-keto-4- methylthiobutyrate (KMTB) from 1,2-dihydroxy-3-keto-5- methylthiopentene (DHK-MTPene). Also down-regulates cell migration mediated by MMP14. Necessary for hepatitis C virus replication in an otherwise non-permissive cell line. 
Sequence Annotation
 METAL 88 88 Iron or nickel (By similarity).
 METAL 90 90 Iron or nickel (By similarity).
 METAL 94 94 Iron or nickel (By similarity).
 METAL 133 133 Iron or nickel (By similarity).  
Keyword
 Alternative splicing; Amino-acid biosynthesis; Cell membrane; Complete proteome; Cytoplasm; Dioxygenase; Direct protein sequencing; Iron; Membrane; Metal-binding; Methionine biosynthesis; Nucleus; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 179 AA 
Protein Sequence
MVQAWYMDDA PGDPRQPHRP DPGRPVGLEQ LRRLGVLYWK LDADKYENDP ELEKIRRERN 60
YSWMDIITIC KDKLPNYEEK IKMFYEEHLH LDDEIRYILD GSGYFDVRDK EDQWIRIFME 120
KGDMVTLPAG IYHRFTVDEK NYTKAMRLFV GEPVWTAYNR PADHFEARGQ YVKFLAQTA 179 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005794; C:Golgi apparatus; IDA:HPA.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0010309; F:acireductone dioxygenase [iron(II)-requiring] activity; TAS:Reactome.
 GO:0005506; F:iron ion binding; IEA:HAMAP.
 GO:0019509; P:L-methionine salvage from methylthioadenosine; IDA:UniProtKB.
 GO:0006595; P:polyamine metabolic process; TAS:Reactome. 
Interpro
 IPR004313; Acireductn_dOase_family.
 IPR027496; MTCBP-1_eukaryotes.
 IPR014710; RmlC-like_jellyroll.
 IPR011051; RmlC_Cupin. 
Pfam
 PF03079; ARD 
SMART
  
PROSITE
  
PRINTS