CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018813
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Putative ATP-dependent RNA helicase DDX12 
Protein Synonyms/Alias
 CHL1-related protein 2; hCHLR2; DEAD/H box protein 12 
Gene Name
 DDX12P 
Gene Synonyms/Alias
 CHLR2; DDX12 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
354DEALAEVKDMEQLLAubiquitination[1]
364EQLLALGKEARACPYubiquitination[1]
545FSSREQPKLAGFQQFubiquitination[1, 2, 3]
615SLSQSTLKFLLLNPAubiquitination[4]
830RSAELQEKMAYLDQTubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 DNA helicase involved in cellular proliferation. Probably required for maintaining the chromosome segregation (By similarity). 
Sequence Annotation
 DOMAIN 28 464 Helicase ATP-binding.
 NP_BIND 63 70 ATP (By similarity).
 MOTIF 412 415 DEAH.  
Keyword
 ATP-binding; Cell cycle; Complete proteome; DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Reference proteome; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 950 AA 
Protein Sequence
MRSGGGCSGS LSLRGPASFK FSGVCPDSRG LAFSVAILPA KKEDFMAELY RVLEAGKIGI 60
FESPTGTGKS LSLICGALSW LRDFEQKKRE EEARLLETGT GPLHDEKDES LCLSSSCEGA 120
AGTPRPAGEP AWVTQFVQKK EERDLVNRLK AEQARRKQRE ERLQQLQHRV QLKYAAKRLR 180
QEEEERENLL RLSREMLETG PEAERPEQLE SGEEELVLAE YESDEEKKVA SGVDEDEDDL 240
EEEHITKIYH CSRTHSQLAQ FVHEVKKSPF GKDVRLVSLG SRQNLCVNED VRSLGSVQLI 300
NNRCVDMQRS RHEKKKGAEE EKPKRRRQEK QAACPFYNHE QMGLLRDEAL AEVKDMEQLL 360
ALGKEARACP YYGSRLAIPA AQLVVLPYQM LLHAATRQAA GIRLQDQVVI IDEAHNLIDT 420
TTSMHSVEVS GSQLCQAHSQ LLQYMERYGK RLKAKNLMYL KQILYLLEKF VAVLGGNIKQ 480
NPNTQSLSQT GMELKTINDF LFQSQIDNIN LFKVQRYCEK SMISRKLFGF TERYGAVFSS 540
REQPKLAGFQ QFLQSLQPRT TEALAAPADE SQASVPQPAS PLMHIEGFLA ALTTANQDGR 600
VILSRQGSLS QSTLKFLLLN PAVHFAQVVK ECRAVVIAGG TMQPVSNFRQ QLLACAGVEA 660
ERVVEFSCGH VIPPDNILPL VICSGVSNQP LEFTFQKRDL PQMMDEVGRI LCNLCGVVSG 720
GVVCFFPSYE YLRQVHAHWE KGGLLGRLAA RKKIFQEPKS AHQVEQVLLA YSRCLQACGQ 780
ERGPVTGALL LSVVGGKMSE GINFSDNLGR CVVMVGMPFP NIRSAELQEK MAYLDQTLPR 840
APGQAHPGKA LVENLCMKAV NQSIGRAIRH QKDFASIVLL DQRYARPPVL AKLPAWIRAR 900
VEVKATFGPA IAAVQKVSPT FFFLRASQPR DHISHCLLSA QFHREKSASS 950 
Gene Ontology
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004003; F:ATP-dependent DNA helicase activity; IEA:InterPro.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW. 
Interpro
 IPR006555; ATP-dep_Helicase_C.
 IPR027076; DDX11/CHL1.
 IPR010614; DEAD_2.
 IPR013020; DNA_helicase_DNA-repair_Rad3.
 IPR014013; Helic_SF1/SF2_ATP-bd_DinG/Rad3.
 IPR006554; Helicase-like_DEXD_c2.
 IPR027417; P-loop_NTPase. 
Pfam
 PF06733; DEAD_2
 PF13307; Helicase_C_2 
SMART
 SM00488; DEXDc2
 SM00491; HELICc2 
PROSITE
 PS00690; DEAH_ATP_HELICASE
 PS51193; HELICASE_ATP_BIND_2 
PRINTS