CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015645
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tubulin alpha-1A chain 
Protein Synonyms/Alias
 Alpha-tubulin 3; Tubulin B-alpha-1; Tubulin alpha-3 chain 
Gene Name
 TUBA1A 
Gene Synonyms/Alias
 TUBA3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
40DGQMPSDKTIGGGDDacetylation[1, 2]
60FSETGAGKHVPRAVFmethylation[3]
60FSETGAGKHVPRAVFubiquitination[4, 5, 6]
96PEQLITGKEDAANNYubiquitination[4, 5, 6]
112RGHYTIGKEIIDLVLubiquitination[4, 5, 6]
124LVLDRIRKLADQCTGubiquitination[5]
163RLSVDYGKKSKLEFSubiquitination[5, 6]
164LSVDYGKKSKLEFSIubiquitination[6]
304EPANQMVKCDPRHGKubiquitination[6]
311KCDPRHGKYMACCLLubiquitination[6]
326YRGDVVPKDVNAAIAubiquitination[4, 6]
336NAAIATIKTKRTIQFubiquitination[4, 5, 6]
338AIATIKTKRTIQFVDubiquitination[5, 6]
352DWCPTGFKVGINYQPubiquitination[6, 7]
370VPGGDLAKVQRAVCMubiquitination[4, 5, 6]
394AWARLDHKFDLMYAKubiquitination[5, 6]
401KFDLMYAKRAFVHWYubiquitination[5, 6]
430EDMAALEKDYEEVGVubiquitination[5]
Reference
 [1] Microtubule acetylation promotes kinesin-1 binding and transport.
 Reed NA, Cai D, Blasius TL, Jih GT, Meyhofer E, Gaertig J, Verhey KJ.
 Curr Biol. 2006 Nov 7;16(21):2166-72. [PMID: 17084703]
 [2] MEC-17 is an alpha-tubulin acetyltransferase.
 Akella JS, Wloga D, Kim J, Starostina NG, Lyons-Abbott S, Morrissette NS, Dougan ST, Kipreos ET, Gaertig J.
 Nature. 2010 Sep 9;467(7312):218-22. [PMID: 20829795]
 [3] A method for the comprehensive proteomic analysis of membrane proteins.
 Wu CC, MacCoss MJ, Howell KE, Yates JR 3rd.
 Nat Biotechnol. 2003 May;21(5):532-8. [PMID: 12692561]
 [4] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
 Xu G, Paige JS, Jaffrey SR.
 Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [7] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. 
Sequence Annotation
 NP_BIND 142 148 GTP (Potential).
 MOD_RES 6 6 Phosphoserine (By similarity).
 MOD_RES 48 48 Phosphoserine (By similarity).
 MOD_RES 83 83 Nitrated tyrosine (By similarity).
 MOD_RES 282 282 Nitrated tyrosine (By similarity).
 MOD_RES 319 319 Phosphotyrosine (By similarity).
 MOD_RES 432 432 Phosphotyrosine (By similarity).
 MOD_RES 439 439 Phosphoserine (By similarity).  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Disease mutation; GTP-binding; Lissencephaly; Microtubule; Nitration; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 451 AA 
Protein Sequence
MRECISIHVG QAGVQIGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDSFN TFFSETGAGK 60
HVPRAVFVDL EPTVIDEVRT GTYRQLFHPE QLITGKEDAA NNYARGHYTI GKEIIDLVLD 120
RIRKLADQCT GLQGFLVFHS FGGGTGSGFT SLLMERLSVD YGKKSKLEFS IYPAPQVSTA 180
VVEPYNSILT THTTLEHSDC AFMVDNEAIY DICRRNLDIE RPTYTNLNRL IGQIVSSITA 240
SLRFDGALNV DLTEFQTNLV PYPRIHFPLA TYAPVISAEK AYHEQLSVAE ITNACFEPAN 300
QMVKCDPRHG KYMACCLLYR GDVVPKDVNA AIATIKTKRT IQFVDWCPTG FKVGINYQPP 360
TVVPGGDLAK VQRAVCMLSN TTAIAEAWAR LDHKFDLMYA KRAFVHWYVG EGMEEGEFSE 420
AREDMAALEK DYEEVGVDSV EGEGEEEGEE Y 451 
Gene Ontology
 GO:0005881; C:cytoplasmic microtubule; IEA:Compara.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005874; C:microtubule; IDA:UniProtKB.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0005200; F:structural constituent of cytoskeleton; IEA:InterPro.
 GO:0005198; F:structural molecule activity; TAS:BHF-UCL.
 GO:0051084; P:'de novo' posttranslational protein folding; TAS:Reactome.
 GO:0051301; P:cell division; TAS:BHF-UCL.
 GO:0030705; P:cytoskeleton-dependent intracellular transport; TAS:BHF-UCL.
 GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:Reactome.
 GO:0007017; P:microtubule-based process; TAS:BHF-UCL.
 GO:0051258; P:protein polymerization; IEA:InterPro. 
Interpro
 IPR002452; Alpha_tubulin.
 IPR008280; Tub_FtsZ_C.
 IPR000217; Tubulin.
 IPR018316; Tubulin/FtsZ_2-layer-sand-dom.
 IPR023123; Tubulin_C.
 IPR017975; Tubulin_CS.
 IPR003008; Tubulin_FtsZ_GTPase. 
Pfam
 PF00091; Tubulin
 PF03953; Tubulin_C 
SMART
 SM00864; Tubulin
 SM00865; Tubulin_C 
PROSITE
 PS00227; TUBULIN 
PRINTS
 PR01162; ALPHATUBULIN.
 PR01161; TUBULIN.