| Tag | Content |
|---|
| CPLM ID | CPLM-007494 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 |
Protein Synonyms/Alias | Oligosaccharyl transferase 64 kDa subunit; Oligosaccharyl transferase subunit OST1; Oligosaccharyl transferase subunit alpha |
Gene Name | OST1 |
Gene Synonyms/Alias | NLT1; YJL002C; J1404 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 275 | FSRLELMKQIQTQNM | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. |
Sequence Annotation | CARBOHYD 18 18 N-linked (GlcNAc...) (Potential).
CARBOHYD 99 99 N-linked (GlcNAc...) (Potential).
CARBOHYD 217 217 N-linked (GlcNAc...) (Potential).
CARBOHYD 336 336 N-linked (GlcNAc...) (Potential).
CARBOHYD 400 400 N-linked (GlcNAc...) (Potential). |
Keyword | Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Glycoprotein; Glycosyltransferase; Membrane; Reference proteome; Signal; Transferase; Transmembrane; Transmembrane helix. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 476 AA |
Protein Sequence | MRQVWFSWIV GLFLCFFNVS SAAQYEPPAT WENVDYKRTI DVSNAYISET IEITIKNIAS 60
EPATEYFTAF ESGIFSKVSF FSAYFTNEAT FLNSQLLANS TTAPGDDGES EIRYGIIQFP 120
NAISPQEEVS LVIKSFYNTV GIPYPEHVGM SEEQHLLWET NRLPLSAYDT KKASFTLIGS 180
SSFEEYHPPN DESLLGKANG NSFEFGPWED IPRFSSNETL AIVYSHNAPL NQVVNLRRDI 240
WLSHWASTIQ FEEYYELTNK AAKLSKGFSR LELMKQIQTQ NMRQTHFVTV LDMLLPEGAT 300
DHYFTDLVGL VSTSHAERDH FFIRPRFPIF GGWNYNFTVG WTNKLSDFLH VSSGSDEKFV 360
ASIPILNGPP DTVYDNVELS VFLPEGAEIF DIDSPVPFTN VSIETQKSYF DLNKGHVKLT 420
FSYRNLISQV ANGQVLIKYD YPKSSFFKKP LSIACYIFTA LMGVFVLKTL NMNVTN 476 |
Gene Ontology | GO:0016021; C:integral to membrane; IEA:UniProtKB-KW. GO:0008250; C:oligosaccharyltransferase complex; IPI:SGD. GO:0004579; F:dolichyl-diphosphooligosaccharide-protein glycotransferase activity; IDA:SGD. GO:0018279; P:protein N-linked glycosylation via asparagine; IPI:SGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |