CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024438
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Large proline-rich protein BAG6 
Protein Synonyms/Alias
 BCL2-associated athanogene 6; HLA-B-associated transcript 3 
Gene Name
 BAG6 
Gene Synonyms/Alias
 BAT3 
Created Date
 July 27, 2013 
Organism
 Sus scrofa (Pig) 
NCBI Taxa ID
 9823 
Lysine Modification
Position
Peptide
Type
References
56SVSIPSEKQRLIYQGubiquitination[1]
1045YLSGMPAKRRKTMQGubiquitination[2, 3, 4]
1048GMPAKRRKTMQGEGPubiquitination[2, 3, 4]
1068EAVSRAAKAAGARPLubiquitination[3]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Chaperone that plays a key role in various processes such as apoptosis, insertion of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane and regulation of chromatin. Acts in part by regulating stability of proteins and their degradation by the proteasome. Participates in endoplasmic reticulum stress-induced apoptosis via its interaction with AIFM1/AIF by regulating AIFM1/AIF stability and preventing its degradation. Also required during spermatogenesis for synaptonemal complex assembly via its interaction with HSPA2, by inhibiting polyubiquitination and subsequent proteasomal degradation of HSPA2. Required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, may play a role in immuno-proteasomes to generate antigenic peptides via targeted degradation, thereby playing a role in antigen presentation in immune response. Key component of the BAG6/BAT3 complex, a cytosolic multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. BAG6/BAT3 acts by facilitating TA membrane proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins and transfers them to ASNA1/TRC40 for targeting to the endoplasmic reticulum membrane (By similarity). 
Sequence Annotation
 DOMAIN 17 92 Ubiquitin-like.
 REPEAT 236 265 1.
 REPEAT 410 438 2.
 REPEAT 569 596 3.
 REPEAT 602 630 4.
 REGION 236 630 4 X 29 AA approximate repeats.
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 960 960 Phosphoserine (By similarity).
 MOD_RES 969 969 Phosphoserine (By similarity).
 MOD_RES 1077 1077 Phosphoserine (By similarity).
 MOD_RES 1113 1113 Phosphoserine (By similarity).  
Keyword
 Acetylation; Alternative splicing; Apoptosis; Chaperone; Chromatin regulator; Complete proteome; Cytoplasm; Differentiation; Immunity; Nucleus; Phosphoprotein; Reference proteome; Repeat; Spermatogenesis; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1128 AA 
Protein Sequence
MEPNDSTSTT MEEPESLEVL VKTLDSQTRT FIVGAQMNVK EFKEHIAASV SIPSEKQRLI 60
YQGRVLQDDK KLQEYNVGGK VIHLVERAPP QTQLPSGASS GTGSASATHG GGPPPGTRGP 120
GASVHDRNAN SYVMVGTFNL PSDGSAVDVH INMEQAPIQS EPRVRLVMAQ HMIRDIQTLL 180
SRMECRGGSQ AQHSQPPSQM PTVAPEPVAL SSQTSESVES EVPPREPMAA EEVEERASAQ 240
SPGLSPSGPA PAGPTPAPET NAPNHPSPAE YVEVLQELQR LESRLQPFLQ RYYEVLGAAA 300
TTDYNNNQEG REEDQRLINL VGESLRLLGN TFVALSDLRC NLACAPPRHL HVVRPMSHYT 360
TPMVLQQAAI PIQINVGTTV TMTGNGTRPP PTPNAEAPPP GPGQASSLAP SSTTVESSTE 420
GAPPPGPAPP PAASHPRVIR ISHQSVEPVV MMHMNIQDSG TQPGGVPSAP TGPLGPTGHG 480
QTLGQQVPGF PTAPTRVVIA RPTPPQSRPS HPGGPPVSGA LPGAGLGTNA SLAQMVSGLV 540
GQLLMQPVLV AQGTPGMAPP PAPATASASA GTTNTATTAG PAPGGPAQPP PPQPSASDLQ 600
FSQLLGNLLG PAGPGAGGPG MTSPTITVAM PGVPAFLQGM TDFLQATQTA APPAPPPPPP 660
PPPPAPEQQT APPPGSPPGG AGSPGGLGPE SLPLEFFTSV VQGVLSSLLG SLGARAGSSE 720
SIAAFIQRLS GSSNIFEPGA DGALGFFGAL LSLLCQNFSM VDVVMLLHGH FQPLQRLQPQ 780
LRSFFHQHYL GGQEPTPGNI RTATHTLITG LEEYVRESFS LVQVQPGVDI IRTNLEFLQE 840
QFNSIAAHVM HCTDSGFGAR LLELCNQGLF ECLALNLHCL GGQQMELAAV INGRIRRMSR 900
GVNPSLVSWL TTMMGLRLQV VLEHMPVGPD AILRYVRRVG DPPQPLPEEP MEVQGSERTS 960
PEPQRENASP APGTTAEEAM SRGPPPAPEG GSRDEQDGAA AETEPWAAAV PPEWVPIIQQ 1020
DIQSQRKVKP QPPLSDAYLS GMPAKRRKTM QGEGPQLLLS EAVSRAAKAA GARPLTSPES 1080
LSRDLEAPEV QESYRQQLRA DIQKRLQEDP NYSPQRFPNA HRAFAEDP 1128 
Gene Ontology
 GO:0071818; C:BAT3 complex; ISS:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0031593; F:polyubiquitin binding; ISS:UniProtKB.
 GO:0070628; F:proteasome binding; ISS:UniProtKB.
 GO:0043022; F:ribosome binding; ISS:UniProtKB.
 GO:0007420; P:brain development; ISS:UniProtKB.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0016568; P:chromatin modification; IEA:UniProtKB-KW.
 GO:0009790; P:embryo development; ISS:UniProtKB.
 GO:0018393; P:internal peptidyl-lysine acetylation; ISS:UniProtKB.
 GO:0042771; P:intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; ISS:UniProtKB.
 GO:0070059; P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress; ISS:UniProtKB.
 GO:0001822; P:kidney development; ISS:UniProtKB.
 GO:0030324; P:lung development; ISS:UniProtKB.
 GO:0032435; P:negative regulation of proteasomal ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
 GO:0050821; P:protein stabilization; ISS:UniProtKB.
 GO:0042981; P:regulation of apoptotic process; ISS:UniProtKB.
 GO:0007283; P:spermatogenesis; ISS:UniProtKB.
 GO:0007130; P:synaptonemal complex assembly; ISS:UniProtKB.
 GO:0071816; P:tail-anchored membrane protein insertion into ER membrane; ISS:UniProtKB.
 GO:0006810; P:transport; IEA:UniProtKB-KW.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; ISS:UniProtKB. 
Interpro
 IPR021925; DUF3538.
 IPR000626; Ubiquitin.
 IPR019954; Ubiquitin_CS.
 IPR019955; Ubiquitin_supergroup. 
Pfam
 PF12057; DUF3538
 PF00240; ubiquitin 
SMART
 SM00213; UBQ 
PROSITE
 PS00299; UBIQUITIN_1
 PS50053; UBIQUITIN_2 
PRINTS