CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006447
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tyrosine-protein phosphatase non-receptor type 11 
Protein Synonyms/Alias
 Protein-tyrosine phosphatase SYP; SH-PTP2; SHP-2; Shp2 
Gene Name
 Ptpn11 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
91HHGQLKEKNGDVIELubiquitination[1]
198TDLVEHYKKNPMVETacetylation[2]
358RVIVMTTKEVERGKSacetylation[3]
486LIDIIREKGVDCDIDacetylation[4]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Dephosphorylates ROCK2 at Tyr-722 resulting in stimulatation of its RhoA binding activity (By similarity). 
Sequence Annotation
 DOMAIN 6 102 SH2 1.
 DOMAIN 112 216 SH2 2.
 DOMAIN 247 525 Tyrosine-protein phosphatase.
 REGION 463 469 Substrate binding (By similarity).
 ACT_SITE 463 463 Phosphocysteine intermediate (By
 BINDING 429 429 Substrate (By similarity).
 BINDING 510 510 Substrate (By similarity).
 MOD_RES 2 2 N-acetylthreonine (By similarity).
 MOD_RES 62 62 Phosphotyrosine.
 MOD_RES 63 63 Phosphotyrosine (By similarity).
 MOD_RES 66 66 Phosphotyrosine.
 MOD_RES 280 280 N6-acetyllysine (By similarity).
 MOD_RES 546 546 Phosphotyrosine; by PDGFR.
 MOD_RES 562 562 Phosphoserine (By similarity).
 MOD_RES 584 584 Phosphotyrosine; by PDGFR.
 MOD_RES 595 595 Phosphoserine (By similarity).  
Keyword
 3D-structure; Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Hydrolase; Phosphoprotein; Protein phosphatase; Reference proteome; Repeat; SH2 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 597 AA 
Protein Sequence
MTSRRWFHPN ITGVEAENLL LTRGVDGSFL ARPSKSNPGD FTLSVRRNGA VTHIKIQNTG 60
DYYDLYGGEK FATLAELVQY YMEHHGQLKE KNGDVIELKY PLNCADPTSE RWFHGHLSGK 120
EAEKLLTEKG KHGSFLVRES QSHPGDFVLS VRTGDDKGES NDGKSKVTHV MIRCQELKYD 180
VGGGERFDSL TDLVEHYKKN PMVETLGTVL QLKQPLNTTR INAAEIESRV RELSKLAETT 240
DKVKQGFWEE FETLQQQECK LLYSRKEGQR QENKNKNRYK NILPFDHTRV VLHDGDPNEP 300
VSDYINANII MPEFETKCNN SKPKKSYIAT QGCLQNTVND FWRMVFQENS RVIVMTTKEV 360
ERGKSKCVKY WPDEYALKEY GVMRVRNVKE SAAHDYTLRE LKLSKVGQAL LQGNTERTVW 420
QYHFRTWPDH GVPSDPGGVL DFLEEVHHKQ ESIVDAGPVV VHCSAGIGRT GTFIVIDILI 480
DIIREKGVDC DIDVPKTIQM VRSQRSGMVQ TEAQYRFIYM AVQHYIETLQ RRIEEEQKSK 540
RKGHEYTNIK YSLVDQTSGD QSPLPPCTPT PPCAEMREDS ARVYENVGLM QQQRSFR 597 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0043234; C:protein complex; IEA:Compara.
 GO:0004726; F:non-membrane spanning protein tyrosine phosphatase activity; ISS:UniProtKB.
 GO:0000187; P:activation of MAPK activity; IMP:MGI.
 GO:0007409; P:axonogenesis; IMP:MGI.
 GO:0000077; P:DNA damage checkpoint; IMP:MGI.
 GO:0048013; P:ephrin receptor signaling pathway; ISS:UniProtKB.
 GO:0042593; P:glucose homeostasis; IMP:MGI.
 GO:0042445; P:hormone metabolic process; IMP:MGI.
 GO:0009755; P:hormone-mediated signaling pathway; IDA:MGI.
 GO:0048609; P:multicellular organismal reproductive process; IMP:MGI.
 GO:0051463; P:negative regulation of cortisol secretion; IMP:MGI.
 GO:0060125; P:negative regulation of growth hormone secretion; IMP:MGI.
 GO:0046676; P:negative regulation of insulin secretion; IMP:MGI.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; IMP:MGI.
 GO:0035265; P:organ growth; IMP:MGI.
 GO:2001275; P:positive regulation of glucose import in response to insulin stimulus; IEA:Compara.
 GO:0046887; P:positive regulation of hormone secretion; IMP:MGI.
 GO:0033628; P:regulation of cell adhesion mediated by integrin; ISS:UniProtKB.
 GO:0040014; P:regulation of multicellular organism growth; IMP:MGI.
 GO:0046825; P:regulation of protein export from nucleus; IMP:MGI.
 GO:0006641; P:triglyceride metabolic process; IMP:MGI. 
Interpro
 IPR000980; SH2.
 IPR000387; Tyr/Dual-sp_Pase.
 IPR016130; Tyr_Pase_AS.
 IPR012152; Tyr_Pase_non-rcpt_typ-6/11.
 IPR000242; Tyr_Pase_rcpt/non-rcpt. 
Pfam
 PF00017; SH2
 PF00102; Y_phosphatase 
SMART
 SM00194; PTPc
 SM00252; SH2 
PROSITE
 PS50001; SH2
 PS00383; TYR_PHOSPHATASE_1
 PS50056; TYR_PHOSPHATASE_2
 PS50055; TYR_PHOSPHATASE_PTP 
PRINTS
 PR00700; PRTYPHPHTASE.
 PR00401; SH2DOMAIN.