CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020250
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 RUN and SH3 domain-containing protein 1 
Protein Synonyms/Alias
 New molecule containing SH3 at the carboxy-terminus; Nesca 
Gene Name
 RUSC1 
Gene Synonyms/Alias
 NESCA 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
484TGQLQEQKKGLLIAVubiquitination[1]
511AARNLVQKAQLGDSRubiquitination[1, 2]
711QSLRGTSKEAASDPSubiquitination[2]
803ENENGALKSRRPSSWubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Putative signaling adapter which may play a role in neuronal differentiation. May be involved in regulation of NGF- dependent neurite outgrowth. Proposed to play a role in neuronal vesicular trafficking, specifically involving pre-synaptic membrane proteins. Seems to be involved in signaling pathways that are regulated by the prolonged activation of MAPK. Can regulate the polyubiquitination of IKBKG and thus may be involved in regulation of the NF-kappa-B pathway. 
Sequence Annotation
 DOMAIN 522 666 RUN.
 DOMAIN 844 902 SH3.
 REGION 470 605 Interaction with TRAF6.
 REGION 606 672 Interaction with IKBKG.  
Keyword
 3D-structure; Alternative splicing; Cell junction; Cell membrane; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Cytoskeleton; Endosome; Golgi apparatus; Membrane; Microtubule; Nucleus; Phosphoprotein; Polymorphism; Postsynaptic cell membrane; Reference proteome; SH3 domain; Synapse; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 902 AA 
Protein Sequence
MLSPQRALLC NLNHIHLQHV SLGLHLSRRP ELQEGPLSTP PPPGDTGGKE SRGPCSGTLV 60
DANSNSPAVP CRCCQEHGPG LENRQDPSQE EEGAASPSDP GCSSSLSSCS DLSPDESPVS 120
VYLRDLPGDE DAHPQPSIIP LEQGSPLASA GPGTCSPDSF CCSPDSCSGA SSSPDPGLDS 180
NCNALTTCQD VPSPGLEEED ERAEQDLPTS ELLEADDGKI DAGKTEPSWK INPIWKIDTE 240
KTKAEWKTTE NNNTGWKNNG NVNSSWKSEP EKFDSGWKTN TRITDSGSKT DAGKIDGGWR 300
SDVSEEPVPH RTITSFHELA QKRKRGPGLP LVPQAKKDRS DWLIVFSPDT ELPPSGSPGG 360
SSAPPREVTT FKELRSRSRA PAPPVPPRDP PVGWALVPPR PPPPPVPPRR KKNRPGLQPI 420
AEGQSEEGRA VSPAAGEEAP AAKEPGAQAG LEVRSSWSFA GVPGAQRLWM AEAQSGTGQL 480
QEQKKGLLIA VSVSVDKIIS HFGAARNLVQ KAQLGDSRLS PDVGHLVLTT LCPALHALVA 540
DGLKPFRKDL ITGQRRSSPW SVVEASVKPG SSTRSLGTLY SQVSRLAPLS SSRSRFHAFI 600
LGLLNTKQLE LWFSSLQEDA GLLSLLYLPT GFFSLARGGC PSLSTELLLL LQPLSVLTFH 660
LDLLFEHHHH LPLGPPQAPA PPGPPPALQQ TMQAMLHFGG RLAQSLRGTS KEAASDPSDS 720
PNLPTPGSWW EQLTQASRVY ASGGTEGFPL SRWAPGRHGT AAEEGAQERP LPTDEMAPGR 780
GLWLGRLFGV PGGPAENENG ALKSRRPSSW LPPTVSVLAL VKRGAPPEMP SPQELEASAP 840
RMVQTHRAVR ALCDHTAARP DQLSFRRGEV LRVITTVDED WLRCGRDGME GLVPVGYTSL 900
VL 902 
Gene Ontology
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0016023; C:cytoplasmic membrane-bounded vesicle; IEA:UniProtKB-SubCell.
 GO:0031410; C:cytoplasmic vesicle; ISS:UniProtKB.
 GO:0005769; C:early endosome; ISS:UniProtKB.
 GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0015630; C:microtubule cytoskeleton; ISS:UniProtKB.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
 GO:0014069; C:postsynaptic density; IEA:UniProtKB-SubCell.
 GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-KW.
 GO:0003779; F:actin binding; ISS:UniProtKB.
 GO:0005070; F:SH3/SH2 adaptor activity; TAS:ProtInc.
 GO:0000209; P:protein polyubiquitination; IDA:UniProtKB. 
Interpro
 IPR004012; Run.
 IPR001452; SH3_domain. 
Pfam
 PF02759; RUN
 PF00018; SH3_1 
SMART
 SM00593; RUN
 SM00326; SH3 
PROSITE
 PS50826; RUN
 PS50002; SH3 
PRINTS