UniProt Accession

 CP7A1_MOUSE; Q64505; Q8BFR7 

Genbank Protein ID

 L23754; AK050020; AK050210; AK050220; AK050260; AL772306 

Genbank Nucleotide ID

 AAA68867.1; BAC34033.1; BAC34123.1; BAC34131.1; BAC34150.1; CAM27235.1 

Protein Name

 Cholesterol 7-alpha-monooxygenase 

Protein Synonyms/Alias

 CYPVII; Cholesterol 7-alpha-hydroxylase; Cytochrome P450 7A1 

Gene Name

 Cyp7a1 

Gene Synonyms/Alias

 Cyp7 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
28	IIGIRRRKVGEPPLD	ubiquitination	[1]
127	NTTENINKTFNKTLQ	ubiquitination	[1]
229	GVPIHLFKTAHKARE	ubiquitination	[1]
275	STFDDMEKAKTHLVI	ubiquitination	[1]
367	SLNIRTAKEDFTLHL	ubiquitination	[1]
418	RYLDESGKAKTTFYR	ubiquitination	[1]
420	LDESGKAKTTFYRNG	ubiquitination	[1]
498	PLNDIEFKYKLKH**	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis. 

Sequence Annotation

 METAL 444 444 Iron (heme axial ligand) (By similarity).  

Keyword

 Cholesterol metabolism; Complete proteome; Endoplasmic reticulum; Heme; Iron; Lipid metabolism; Membrane; Metal-binding; Microsome; Monooxygenase; NADP; Oxidoreductase; Reference proteome; Steroid metabolism; Sterol metabolism. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 503 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0043231; C:intracellular membrane-bounded organelle; ISS:UniProtKB.
 GO:0008123; F:cholesterol 7-alpha-monooxygenase activity; ISS:UniProtKB.
 GO:0009055; F:electron carrier activity; IEA:InterPro.
 GO:0020037; F:heme binding; IEA:InterPro.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0006699; P:bile acid biosynthetic process; ISS:UniProtKB.
 GO:0071397; P:cellular response to cholesterol; ISS:UniProtKB.
 GO:0071333; P:cellular response to glucose stimulus; ISS:UniProtKB.
 GO:0006707; P:cholesterol catabolic process; ISS:UniProtKB.
 GO:0042632; P:cholesterol homeostasis; ISS:UniProtKB.
 GO:0070859; P:positive regulation of bile acid biosynthetic process; TAS:BHF-UCL. 

Interpro

 IPR001128; Cyt_P450.
 IPR017972; Cyt_P450_CS.
 IPR024204; Cyt_P450_CYP7A1-type.
 IPR002403; Cyt_P450_E_grp-IV. 

Pfam

 PF00067; p450 

SMART

  

PROSITE

 PS00086; CYTOCHROME_P450 

PRINTS

 PR00465; EP450IV.