UniProt Accession

 MCA3_HUMAN; O43324; C9JLK5; Q5THS2 

Genbank Protein ID

 AB011079; AF054186; BT007306; AL355499; AL023694; AL451187; AL023694; AL355499; AL451187; AL451187; AL023694; AL355499; BC005291; CK002875 

Genbank Nucleotide ID

 BAA24926.1; AAC39916.1; AAP35970.1; CAH72539.1; CAH72539.1; CAH72539.1; CAI21644.1; CAI21644.1; CAI21644.1; CAI14747.1; CAI14747.1; CAI14747.1; AAH05291.1 

Protein Name

 Eukaryotic translation elongation factor 1 epsilon-1 

Protein Synonyms/Alias

 Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3; Elongation factor p18; Multisynthase complex auxiliary component p18 

Gene Name

 EEF1E1 

Gene Synonyms/Alias

 AIMP3; P18 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
12	AELSLLEKSLGLSKG	acetylation	[1]
12	AELSLLEKSLGLSKG	ubiquitination	[2, 3, 4]
18	EKSLGLSKGNKYSAQ	ubiquitination	[3]
53	TIAAHLVKQANKEYL	ubiquitination	[5]
57	HLVKQANKEYLLGST	ubiquitination	[3, 5]
88	QVDGHSSKNDIHTLL	ubiquitination	[5]
96	NDIHTLLKDLNSYLE	ubiquitination	[5]
138	LTVQEKEKYLNVSRW	acetylation	[1, 6]
166	LSSVVFIKNRLYTNS	acetylation	[1]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Positive modulator of ATM response to DNA damage. 

Sequence Annotation

 DOMAIN 50 173 GST C-terminal.
 REGION 2 56 N-terminal.
 REGION 57 63 Linker.
 REGION 64 152 C-terminal.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 138 138 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleus; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 174 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0008285; P:negative regulation of cell proliferation; ISS:UniProtKB.
 GO:0043065; P:positive regulation of apoptotic process; ISS:UniProtKB.
 GO:0043517; P:positive regulation of DNA damage response, signal transduction by p53 class mediator; ISS:UniProtKB.
 GO:0006418; P:tRNA aminoacylation for protein translation; TAS:Reactome. 

Interpro

 IPR010987; Glutathione-S-Trfase_C-like.
 IPR017933; Glutathione_S_Trfase/Cl_chnl_C.
 IPR004046; GST_C. 

Pfam

 PF00043; GST_C 

SMART

  

PROSITE

 PS50405; GST_CTER 

PRINTS