CPLM-000277

CPLM 1.0 - Compendium of Protein Lysine Modification

Tag

Content

CPLM ID

CPLM-000277

UniProt Accession

TRDMT_HUMAN; O14717; B0YJ02; B0YJ03; B0YJ07; B0YJ08; O43669; Q86WW6

Genbank Protein ID

AF012128; AJ223333; AF045888; EF444974; EF444974; EF444974; EF444974; AL133415; AC067747; CH471072; CH471072; BC047733; AF329940; AF329941; AF329942; AF329943; AF329944

Genbank Nucleotide ID

AAC51939.1; CAA11272.1; AAC39764.1; ACA05980.1; ACA05984.1; ACA05985.1; ACA05986.1; CAB87964.1; CAB87964.1; EAW86218.1; EAW86219.1; AAH47733.1; AAK68034.1; AAK68035.1; AAK68036.1; AAK68037.1; AAK68033.1

Protein Name

tRNA (cytosine(38)-C(5))-methyltransferase

Protein Synonyms/Alias

DNA (cytosine-5)-methyltransferase-like protein 2; Dnmt2; DNA methyltransferase homolog HsaIIP; DNA MTase homolog HsaIIP; M.HsaIIP; PuMet

Gene Name

TRDMT1

Gene Synonyms/Alias

DNMT2

Created Date

July 27, 2013

Organism

Homo sapiens (Human)

NCBI Taxa ID

9606

Lysine Modification

Position	Peptide	Type	References
122	YILLENVKGFEVSST	ubiquitination	[1]
204	YAMDVENKIQEKNVE	ubiquitination	[1]

Reference

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]

Functional Description

Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp).

Sequence Annotation

ACT_SITE 79 79 By similarity.

Keyword

3D-structure; Alternative splicing; Complete proteome; Methyltransferase; Nucleus; Polymorphism; Reference proteome; RNA-binding; S-adenosyl-L-methionine; Transferase; tRNA processing.

Sequence Source

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

Protein Length

391 AA

Protein Sequence

MEPLRVLELY SGVGGMHHAL RESCIPAQVV AAIDVNTVAN EVYKYNFPHT QLLAKTIEGI 60
TLEEFDRLSF DMILMSPPCQ PFTRIGRQGD MTDSRTNSFL HILDILPRLQ KLPKYILLEN 120
VKGFEVSSTR DLLIQTIENC GFQYQEFLLS PTSLGIPNSR LRYFLIAKLQ SEPLPFQAPG 180
QVLMEFPKIE SVHPQKYAMD VENKIQEKNV EPNISFDGSI QCSGKDAILF KLETAEEIHR 240
KNQQDSDLSV KMLKDFLEDD TDVNQYLLPP KSLLRYALLL DIVQPTCRRS VCFTKGYGSY 300
IEGTGSVLQT AEDVQVENIY KSLTNLSQEE QITKLLILKL RYFTPKEIAN LLGFPPEFGF 360
PEKITVKQRY RLLGNSLNVH VVAKLIKILY E 391

Gene Ontology

GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0003886; F:DNA (cytosine-5-)-methyltransferase activity; TAS:ProtInc.
GO:0003677; F:DNA binding; IEA:InterPro.
GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
GO:0001975; P:response to amphetamine; IEA:Compara.
GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.

Interpro

IPR018117; C5_DNA_meth_AS.
IPR001525; C5_MeTfrase.
IPR025813; DNA/tRNA_C5-MeTrfase.

Pfam

PF00145; DNA_methylase

SMART

PROSITE

PS00094; C5_MTASE_1
PS00095; C5_MTASE_2

PRINTS

PR00105; C5METTRFRASE.