| Tag | Content |
|---|
| CPLM ID | CPLM-000628 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase |
Protein Synonyms/Alias | UDP-GlcNAc-2-epimerase/ManAc kinase; UDP-N-acetylglucosamine 2-epimerase (hydrolyzing); UDP-GlcNAc-2-epimerase; Uridine diphosphate-N-acetylglucosamine-2-epimerase; N-acetylmannosamine kinase; ManAc kinase |
Gene Name | Gne |
Gene Synonyms/Alias | Glcne |
Created Date | July 27, 2013 |
Organism | Rattus norvegicus (Rat) |
NCBI Taxa ID | 10116 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 195 | KLLSAKNKDYMSIIR | ubiquitination | [1] |
|
Reference | [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis. Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J. J Proteome Res. 2012 Sep 7;11(9):4722-32. [ PMID: 22871113] |
Functional Description | Regulates and initiates biosynthesis of N- acetylneuraminic acid (NeuAc), a precursor of sialic acids. Required for normal sialylation in hematopoietic cells. Sialylation is implicated in cell adhesion, signal transduction, tumorigenicity and metastatic behavior of malignant cells. Plays an essential role in early development (By similarity). |
Sequence Annotation | NP_BIND 411 420 ATP (By similarity).
NP_BIND 543 552 ATP (By similarity).
REGION 1 ? UDP-N-acetylglucosamine 2-epimerase.
REGION 406 722 N-acetylmannosamine kinase (By
ACT_SITE 517 517 By similarity.
METAL 569 569 Zinc (By similarity).
METAL 579 579 Zinc (By similarity).
METAL 581 581 Zinc (By similarity).
METAL 586 586 Zinc (By similarity).
BINDING 477 477 Substrate (By similarity).
BINDING 489 489 Substrate (By similarity).
BINDING 517 517 Substrate (By similarity).
BINDING 566 566 Substrate (By similarity).
BINDING 569 569 Substrate (By similarity).
BINDING 588 588 Substrate (By similarity). |
Keyword | Allosteric enzyme; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Metal-binding; Multifunctional enzyme; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase; Zinc. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 722 AA |
Protein Sequence | MEKNGNNRKL RVCVATCNRA DYSKLAPIMF GIKTEPAFFE LDVVVLGSHL IDDYGNTYRM 60
IEQDDFDINT RLHTIVRGED EAAMVESVGL ALVKLPDVLN RLKPDIMIVH GDRFDALALA 120
TSAALMNIRI LHIEGGEVSG TIDDSIRHAI TKLAHYHVCC TRSAEQHLIS MCEDHDRILL 180
AGCPSYDKLL SAKNKDYMSI IRMWLGDDVK CKDYIVALQH PVTTDIKHSI KMFELTLDAL 240
ISFNKRTLVL FPNIDAGSKE MVRVMRKKGI EHHPNFRAVK HVPFDQFIQL VAHAGCMIGN 300
SSCGVREVGA FGTPVINLGT RQIGRETGEN VLHVRDADTQ DKILQALHLQ FGKQYPCSKI 360
YGDGNAVPRI LKFLKSIDLQ EPLQKKFCFP PVKENISQDI DHILETLSAL AVDLGGTNLR 420
VAIVSMKGEI VKKYTQFNPK TYEERISLIL QMCVEAAAEA VKLNCRILGV GISTGGRVNP 480
QEGVVLHSTK LIQEWNSVDL RTPLSDTLHL PVWVDNDGNC AAMAERKFGQ GKGQENFVTL 540
ITGTGIGGGI IHQHELIHGS SFCAAELGHL VVSLDGPDCS CGSHGCIEAY ASGMALQREA 600
KKLHDEDLLL VEGMSVPKDE AVGALHLIQA AKLGNVKAQS ILRTAGTALG LGVVNILHTM 660
NPSLVILSGV LASHYIHIVR DVIRQQALSS VQDVDVVVSD LVDPALLGAA SMVLDYTTRR 720
IH 722 |
Gene Ontology | GO:0005829; C:cytosol; IDA:RGD. GO:0005524; F:ATP binding; IEA:UniProtKB-KW. GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. GO:0009384; F:N-acylmannosamine kinase activity; IDA:RGD. GO:0008761; F:UDP-N-acetylglucosamine 2-epimerase activity; IDA:RGD. GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:InterPro. GO:0006045; P:N-acetylglucosamine biosynthetic process; IEA:UniProtKB-UniPathway. GO:0046380; P:N-acetylneuraminate biosynthetic process; TAS:RGD. GO:0006047; P:UDP-N-acetylglucosamine metabolic process; IEA:InterPro. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |