CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009288
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Calcineurin B homologous protein 1 
Protein Synonyms/Alias
 Calcineurin B-like protein; Calcium-binding protein CHP; Calcium-binding protein p22; EF-hand calcium-binding domain-containing protein p22; Sid 470; p24 
Gene Name
 Chp1 
Gene Synonyms/Alias
 Chp; Sid470 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
40SRFTSLDKGENGTLSubiquitination[1]
100IEDNEKSKDVNGPEPubiquitination[1]
114PLNSRSNKLHFAFRLubiquitination[1]
182EFVKVLEKVDVEQKMubiquitination[1]
188EKVDVEQKMSIRFLHubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Calcium-binding protein involved in different processes such as regulation of vesicular trafficking, plasma membrane Na(+)/H(+) exchanger and gene transcription. Involved in the constitutive exocytic membrane traffic. Mediates the association between microtubules and membrane-bound organelles of the endoplasmic reticulum and Golgi apparatus and is also required for the targeting and fusion of transcytotic vesicles (TCV) with the plasma membrane. Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na(+)/H(+) exchange activity. Affects the pH sensitivity of SLC9A1/NHE1 by increasing its sensitivity at acidic pH. Required for the stabilization and localization of SLC9A1/NHE1 at the plasma membranes. Inhibits serum- and GTPase-stimulated Na(+)/H(+) exchange. Plays a role as an inhibitor of ribosomal RNA transcription by repressing the nucleolar UBF1 transcriptional activity. May sequester UBF1 in the nucleoplasm and limit its translocation to the nucleolus. Associates to the ribosomal gene promoter. Acts as a negative regulator of the calcineurin/NFAT signaling pathway. Inhibits NFAT nuclear translocation and transcriptional activity by suppressing the calcium-dependent calcineurin phosphatase activity. Also negatively regulates the kinase activity of the apoptosis-induced kinase STK17B. Inhibits both STK17B auto- and substrate- phosphorylations in a calcium-dependent manner (By similarity). 
Sequence Annotation
 DOMAIN 26 61 EF-hand 1.
 DOMAIN 71 106 EF-hand 2.
 DOMAIN 110 145 EF-hand 3.
 DOMAIN 151 186 EF-hand 4.
 MOTIF 2 6 Necessary for association with
 MOTIF 138 147 Nuclear export signal 1 (By similarity).
 MOTIF 176 185 Nuclear export signal 2 (By similarity).
 LIPID 2 2 N-myristoyl glycine (Probable).  
Keyword
 Calcium; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Endoplasmic reticulum; Lipoprotein; Membrane; Metal-binding; Myristate; Nucleus; Phosphoprotein; Protein kinase inhibitor; Protein transport; Reference proteome; Repeat; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 195 AA 
Protein Sequence
MGSRASTLLR DEELEEIKKE TGFSHSQITR LYSRFTSLDK GENGTLSRED FQRIPELAIN 60
PLGDRIINAF FSEGEDQVNF RGFMRTLAHF RPIEDNEKSK DVNGPEPLNS RSNKLHFAFR 120
LYDLDKDDKI SRDELLQVLR MMVGVNISDE QLGSIADRTI QEADQDGDSA ISFTEFVKVL 180
EKVDVEQKMS IRFLH 195 
Gene Ontology
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
 GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IEA:UniProtKB-SubCell.
 GO:0000139; C:Golgi membrane; ISS:UniProtKB.
 GO:0015630; C:microtubule cytoskeleton; ISS:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0005886; C:plasma membrane; ISS:UniProtKB.
 GO:0030133; C:transport vesicle; ISS:UniProtKB.
 GO:0005509; F:calcium ion binding; ISS:UniProtKB.
 GO:0048306; F:calcium-dependent protein binding; ISS:UniProtKB.
 GO:0019900; F:kinase binding; ISS:UniProtKB.
 GO:0008017; F:microtubule binding; ISS:UniProtKB.
 GO:0004860; F:protein kinase inhibitor activity; IEA:UniProtKB-KW.
 GO:0005215; F:transporter activity; ISS:UniProtKB.
 GO:0017156; P:calcium ion-dependent exocytosis; IEA:Compara.
 GO:0071468; P:cellular response to acidity; ISS:UniProtKB.
 GO:0031122; P:cytoplasmic microtubule organization; ISS:UniProtKB.
 GO:0022406; P:membrane docking; ISS:UniProtKB.
 GO:0061025; P:membrane fusion; ISS:UniProtKB.
 GO:0001578; P:microtubule bundle formation; ISS:UniProtKB.
 GO:0070885; P:negative regulation of calcineurin-NFAT signaling cascade; ISS:UniProtKB.
 GO:0032088; P:negative regulation of NF-kappaB transcription factor activity; ISS:UniProtKB.
 GO:0010923; P:negative regulation of phosphatase activity; ISS:UniProtKB.
 GO:0031953; P:negative regulation of protein autophosphorylation; ISS:UniProtKB.
 GO:0042308; P:negative regulation of protein import into nucleus; ISS:UniProtKB.
 GO:0006469; P:negative regulation of protein kinase activity; ISS:UniProtKB.
 GO:0031397; P:negative regulation of protein ubiquitination; ISS:UniProtKB.
 GO:0060050; P:positive regulation of protein glycosylation; ISS:UniProtKB.
 GO:0090314; P:positive regulation of protein targeting to membrane; ISS:UniProtKB.
 GO:0032417; P:positive regulation of sodium:hydrogen antiporter activity; ISS:UniProtKB.
 GO:0006611; P:protein export from nucleus; ISS:UniProtKB.
 GO:0051259; P:protein oligomerization; IEA:Compara.
 GO:0050821; P:protein stabilization; ISS:UniProtKB.
 GO:0051453; P:regulation of intracellular pH; ISS:UniProtKB.
 GO:0045056; P:transcytosis; IEA:Compara. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom. 
Pfam
 PF13405; EF_hand_4
 PF13499; EF_hand_5 
SMART
 SM00054; EFh 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2 
PRINTS