CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008426
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 H(+)/Cl(-) exchange transporter 5 
Protein Synonyms/Alias
 Chloride channel protein 5; ClC-5; Chloride transporter ClC-5 
Gene Name
 CLCN5 
Gene Synonyms/Alias
 CLCK2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
57DREVGEDKSYNGGGIubiquitination[1, 2]
649AKEEFAHKTLAMDVMubiquitination[3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons. Important for normal acidification of the endosome lumen. May play an important role in renal tubular function. 
Sequence Annotation
 DOMAIN 586 650 CBS 1.
 DOMAIN 682 742 CBS 2.
 NP_BIND 617 619 ATP.
 NP_BIND 724 727 ATP.
 MOTIF 167 171 Selectivity filter part_1 (By
 MOTIF 209 213 Selectivity filter part_2 (By
 MOTIF 453 457 Selectivity filter part_3 (By
 BINDING 168 168 Chloride (By similarity).
 BINDING 455 455 Chloride; via amide nitrogen (By
 BINDING 558 558 Chloride (By similarity).
 BINDING 596 596 ATP; via amide nitrogen and carbonyl  
Keyword
 3D-structure; Alternative splicing; Antiport; ATP-binding; CBS domain; Cell membrane; Chloride; Complete proteome; Disease mutation; Endosome; Golgi apparatus; Ion transport; Membrane; Nucleotide-binding; Polymorphism; Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 746 AA 
Protein Sequence
MAMWQGAMDN RGFQQGSFSS FQNSSSDEDL MDIPATAMDF SMRDDVPPLD REVGEDKSYN 60
GGGIGSSNRI MDFLEEPIPG VGTYDDFNTI DWVREKSRDR DRHREITNKS KESTWALIHS 120
VSDAFSGWLL MLLIGLLSGS LAGLIDISAH WMTDLKEGIC TGGFWFNHEH CCWNSEHVTF 180
EERDKCPEWN SWSQLIISTD EGAFAYIVNY FMYVLWALLF AFLAVSLVKV FAPYACGSGI 240
PEIKTILSGF IIRGYLGKWT LVIKTITLVL AVSSGLSLGK EGPLVHVACC CGNILCHCFN 300
KYRKNEAKRR EVLSAAAAAG VSVAFGAPIG GVLFSLEEVS YYFPLKTLWR SFFAALVAAF 360
TLRSINPFGN SRLVLFYVEF HTPWHLFELV PFILLGIFGG LWGALFIRTN IAWCRKRKTT 420
QLGKYPVIEV LVVTAITAIL AFPNEYTRMS TSELISELFN DCGLLDSSKL CDYENRFNTS 480
KGGELPDRPA GVGVYSAMWQ LALTLILKIV ITIFTFGMKI PSGLFIPSMA VGAIAGRLLG 540
VGMEQLAYYH QEWTVFNSWC SQGADCITPG LYAMVGAAAC LGGVTRMTVS LVVIMFELTG 600
GLEYIVPLMA AAMTSKWVAD ALGREGIYDA HIRLNGYPFL EAKEEFAHKT LAMDVMKPRR 660
NDPLLTVLTQ DSMTVEDVET IISETTYSGF PVVVSRESQR LVGFVLRRDL IISIENARKK 720
QDGVVSTSII YFTEHSPPLP PYTPPTLKLR NILDLSPFTV TDLTPMEIVV DIFRKLGLRQ 780
CLVTHNGRLL GIITKKDVLK HIAQMANQDP DSILFN 816 
Gene Ontology
 GO:0045177; C:apical part of cell; IDA:UniProtKB.
 GO:0010008; C:endosome membrane; TAS:Reactome.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005254; F:chloride channel activity; TAS:ProtInc.
 GO:0005247; F:voltage-gated chloride channel activity; IEA:InterPro.
 GO:0006897; P:endocytosis; IEA:Compara.
 GO:0007588; P:excretion; TAS:ProtInc. 
Interpro
 IPR014743; Cl-channel_core.
 IPR001807; Cl-channel_volt-gated.
 IPR002247; Cl_channel-5.
 IPR000644; Cysta_beta_synth_core. 
Pfam
 PF00571; CBS
 PF00654; Voltage_CLC 
SMART
 SM00116; CBS 
PROSITE
 PS51371; CBS 
PRINTS
 PR00762; CLCHANNEL.
 PR01116; CLCHANNEL5.