CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011747
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Midasin 
Protein Synonyms/Alias
 MIDAS-containing protein; Ribosome export/assembly protein 1 
Gene Name
 MDN1 
Gene Synonyms/Alias
 REA1; YLR106C; L8004.13 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
1103LADITGHKFVRINNHacetylation[1]
1924TLEDQVCKRKLWGNSubiquitination[2]
1995IFGKFSTKENFFKLTacetylation[1]
1995IFGKFSTKENFFKLTubiquitination[2]
2122EIISMNMKLSPNATAubiquitination[3]
2931YDLFLKTKTFSQNLMubiquitination[2]
3455NARLDLVKAFYKHIQacetylation[1]
3486IKFYYQFKPLIDERIacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 
Functional Description
 Nuclear chaperone required for maturation and nuclear export of pre-60S ribosome subunits. Functions at successive maturation steps to remove ribosomal factors at critical transition points, first driving the exit of early pre-60S particles from the nucleolus and then driving late pre-60S particles from the nucleus. Mediates ATP-dependent remodeling of the pre-ribosome just prior to export to allow maturation of 60S subunits into export-competent particles. Removes the ribosome biogenesis factor RSA4 from pre-60S ribosomal subunits in the nucleoplasm to drive nuclear export of the subunit. Involved in 3' processing of the 5.8S rRNA. 
Sequence Annotation
 DOMAIN 4704 4899 VWFA.
 NP_BIND 315 322 ATP (Potential).
 NP_BIND 653 660 ATP (Potential).
 NP_BIND 1083 1090 ATP (Potential).
 NP_BIND 1368 1375 ATP (Potential).
 NP_BIND 1747 1754 ATP (Potential).
 NP_BIND 2054 2061 ATP (Potential).
 MOD_RES 1026 1026 Phosphothreonine.
 MOD_RES 2971 2971 Phosphoserine.
 MOD_RES 4353 4353 Phosphoserine.
 MOD_RES 4388 4388 Phosphothreonine.
 MOD_RES 4555 4555 Phosphoserine.  
Keyword
 ATP-binding; Chaperone; Complete proteome; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 4910 AA 
Protein Sequence
MSQDRILLDL DVVNQRLILF NSAFPSDAIE APFHFSNKES TSENLDNLAG TILHSRSITG 60
HVFLYKHIFL EIVARWIKDS KKKDYVLVIE KLASIITIFP VAMPLIEDYL DKENDHFITI 120
LQNPSTQKDS DMFKILLAYY RLLYHNKEVF ARFIQPDILY QLVDLLTKEQ ENQVVIFLAL 180
KVLSLYLDMG EKTLNDMLDT YIKSRDSLLG HFEGDSGIDY SFLELNEAKR CANFSKLPSV 240
PECFTIEKKS SYFIIEPQDL STKVASICGV IVPKVHTIHD KVFYPLTFVP THKTVSSLRQ 300
LGRKIQNSTP IMLIGKAGSG KTFLINELSK YMGCHDSIVK IHLGEQTDAK LLIGTYTSGD 360
KPGTFEWRAG VLATAVKEGR WVLIEDIDKA PTDVLSILLS LLEKRELTIP SRGETVKAAN 420
GFQLISTVRI NEDHQKDSSN KIYNLNMIGM RIWNVIELEE PSEEDLTHIL AQKFPILTNL 480
IPKLIDSYKN VKSIYMNTKF ISLNKGAHTR VVSVRDLIKL CERLDILFKN NGINKPDQLI 540
QSSVYDSIFS EAADCFAGAI GEFKALEPII QAIGESLDIA SSRISLFLTQ HVPTLENLDD 600
SIKIGRAVLL KEKLNIQKKS MNSTLFAFTN HSLRLMEQIS VCIQMTEPVL LVGETGTGKT 660
TVVQQLAKML AKKLTVINVS QQTETGDLLG GYKPVNSKTV AVPIQENFET LFNATFSLKK 720
NEKFHKMLHR CFNKNQWKNV VKLWNEAYKM AQSILKITNT ENENENAKKK KRRLNTHEKK 780
LLLDKWADFN DSVKKFEAQS SSIENSFVFN FVEGSLVKTI RAGEWLLLDE VNLATADTLE 840
SISDLLTEPD SRSILLSEKG DAEPIKAHPD FRIFACMNPA TDVGKRDLPM GIRSRFTEIY 900
VHSPERDITD LLSIIDKYIG KYSVSDEWVG NDIAELYLEA KKLSDNNTIV DGSNQKPHFS 960
IRTLTRTLLY VTDIIHIYGL RRSLYDGFCM SFLTLLDQKS EAILKPVIEK FTLGRLKNVK 1020
SIMSQTPPSP GPDYVQFKHY WMKKGPNTIQ EQAHYIITPF VEKNMMNLVR ATSGKRFPVL 1080
IQGPTSSGKT SMIKYLADIT GHKFVRINNH EHTDLQEYLG TYVTDDTGKL SFKEGVLVEA 1140
LRKGYWIVLD ELNLAPTDVL EALNRLLDDN RELFIPETQE VVHPHPDFLL FATQNPPGIY 1200
GGRKILSRAF RNRFLELHFD DIPQDELEII LRERCQIAPS YAKKIVEVYR QLSIERSASR 1260
LFEQKNSFAT LRDLFRWALR DAVGYEQLAA SGYMLLAERC RTPQEKVTVK KTLEKVMKVK 1320
LDMDQYYASL EDKSLEAIGS VTWTKGMRRL SVLVSSCLKN KEPVLLVGET GCGKTTICQL 1380
LAQFMGRELI TLNAHQNTET GDILGAQRPV RNRSEIQYKL IKSLKTALNI ANDQDVDLKE 1440
LLQLYSKSDN KNIAEDVQLE IQKLRDSLNV LFEWSDGPLI QAMRTGNFFL LDEISLADDS 1500
VLERLNSVLE PERSLLLAEQ GSSDSLVTAS ENFQFFATMN PGGDYGKKEL SPALRNRFTE 1560
IWVPSMEDFN DVNMIVSSRL LEDLKDLANP IVKFSEWFGK KLGGGNATSG VISLRDILAW 1620
VEFINKVFPK IQNKSTALIQ GASMVFIDAL GTNNTAYLAE NENDLKSLRT ECIIQLLKLC 1680
GDDLELQQIE TNEIIVTQDE LQVGMFKIPR FPDAQSSSFN LTAPTTASNL VRVVRAMQVH 1740
KPILLEGSPG VGKTSLITAL ANITGNKLTR INLSEQTDLV DLFGADAPGE RSGEFLWHDA 1800
PFLRAMKKGE WVLLDEMNLA SQSVLEGLNA CLDHRGEAYI PELDISFSCH PNFLVFAAQN 1860
PQYQGGGRKG LPKSFVNRFS VVFIDMLTSD DLLLIAKHLY PSIEPDIIAK MIKLMSTLED 1920
QVCKRKLWGN SGSPWEFNLR DTLRWLKLLN QYSICEDVDV FDFVDIIVKQ RFRTISDKNK 1980
AQLLIEDIFG KFSTKENFFK LTEDYVQINN EVALRNPHYR YPITQNLFPL ECNVAVYESV 2040
LKAINNNWPL VLVGPSNSGK TETIRFLASI LGPRVDVFSM NSDIDSMDIL GGYEQVDLTR 2100
QISYITEELT NIVREIISMN MKLSPNATAI MEGLNLLKYL LNNIVTPEKF QDFRNRFNRF 2160
FSHLEGHPLL KTMSMNIEKM TEIITKEASV KFEWFDGMLV KAVEKGHWLI LDNANLCSPS 2220
VLDRLNSLLE IDGSLLINEC SQEDGQPRVL KPHPNFRLFL TMDPKYGELS RAMRNRGVEI 2280
YIDELHSRST AFDRLTLGFE LGENIDFVSI DDGIKKIKLN EPDMSIPLKH YVPSYLSRPC 2340
IFAQVHDILL LSDEEPIEES LAAVIPISHL GEVGKWANNV LNCTEYSEKK IAERLYVFIT 2400
FLTDMGVLEK INNLYKPANL KFQKALGLHD KQLTEETVSL TLNEYVLPTV SKYSDKIKSP 2460
ESLYLLSSLR LLLNSLNALK LINEKSTHGK IDELTYIELS AAAFNGRHLK NIPRIPIFCI 2520
LYNILTVMSE NLKTESLFCG SNQYQYYWDL LVIVIAALET AVTKDEARLR VYKELIDSWI 2580
ASVKSKSDIE ITPFLNINLE FTDVLQLSRG HSITLLWDIF RKNYPTTSNS WLAFEKLINL 2640
SEKFDKVRLL QFSESYNSIK DLMDVFRLLN DDVLNNKLSE FNLLLSKLED GINELELISN 2700
KFLNKRKHYF ADEFDNLIRY TFSVDTAELI KELAPASSLA TQKLTKLITN KYNYPPIFDV 2760
LWTEKNAKLT SFTSTIFSSQ FLEDVVRKSN NLKSFSGNQI KQSISDAELL LSSTIKCSPN 2820
LLKSQMEYYK NMLLSWLRKV IDIHVGGDCL KLTLKELCSL IEEKTASETR VTFAEYIFPA 2880
LDLAESSKSL EELGEAWITF GTGLLLLFVP DSPYDPAIHD YVLYDLFLKT KTFSQNLMKS 2940
WRNVRKVISG DEEIFTEKLI NTISDDDAPQ SPRVYRTGMS IDSLFDEWMA FLSSTMSSRQ 3000
IKELVSSYKC NSDQSDRRLE MLQQNSAHFL NRLESGYSKF ADLNDILAGY IYSINFGFDL 3060
LKLQKSKDRA SFQISPLWSM DPINISCAEN VLSAYHELSR FFKKGDMEDT SIEKVLMYFL 3120
TLFKFHKRDT NLLEIFEAAL YTLYSRWSVR RFRQEQEENE KSNMFKFNDN SDDYEADFRK 3180
LFPDYEDTAL VTNEKDISSP ENLDDIYFKL ADTYISVFDK DHDANFSSEL KSGAIITTIL 3240
SEDLKNTRIE ELKSGSLSAV INTLDAETQS FKNTEVFGNI DFYHDFSIPE FQKAGDIIET 3300
VLKSVLKLLK QWPEHATLKE LYRVSQEFLN YPIKTPLARQ LQKIEQIYTY LAEWEKYASS 3360
EVSLNNTVKL ITDLIVSWRK LELRTWKGLF NSEDAKTRKS IGKWWFYLYE SIVISNFVSE 3420
KKETAPNATL LVSSLNLFFS KSTLGEFNAR LDLVKAFYKH IQLIGLRSSK IAGLLHNTIK 3480
FYYQFKPLID ERITNGKKSL EKEIDDIILL ASWKDVNVDA LKQSSRKSHN NLYKIVRKYR 3540
DLLNGDAKTI IEAGLLYSNE NKLKLPTLKQ HFYEDPNLEA SKNLVKEIST WSMRAAPLRN 3600
IDTVASNMDS YLEKISSQEF PNFADLASDF YAEAERLRKE TPNVYTKENK KRLAYLKTQK 3660
SKLLGDALKE LRRIGLKVNF REDIQKVQSS TTTILANIAP FNNEYLNSSD AFFFKILDLL 3720
PKLRSAASNP SDDIPVAAIE RGMALAQSLM FSLITVRHPL SEFTNDYCKI NGMMLDLEHF 3780
TCLKGDIVHS SLKANVDNVR LFEKWLPSLL DYAAQTLSVI SKYSATSEQQ KILLDAKSTL 3840
SSFFVHFNSS RIFDSSFIES YSRFELFINE LLKKLENAKE TGNAFVFDII IEWIKANKGG 3900
PIKKEQKRGP SVEDVEQAFR RTFTSIILSF QKVIGDGIES ISETDDNWLS ASFKKVMVNV 3960
KLLRSSVVSK NIETALSLLK DFDFTTTESI YVKSVISFTL PVITRYYNAM TVVLERSRIY 4020
YTNTSRGMYI LSTILHSLAK NGFCSPQPPS EEVDDKNLQE GTGLGDGEGA QNNNKDVEQD 4080
EDLTEDAQNE NKEQQDKDER DDENEDDAVE MEGDMAGELE DLSNGEENDD EDTDSEEEEL 4140
DEEIDDLNED DPNAIDDKMW DDKASDNSKE KDTDQNLDGK NQEEDVQAAE NDEQQRDNKE 4200
GGDEDPNAPE DGDEEIENDE NAEEENDVGE QEDEVKDEEG EDLEANVPEI ETLDLPEDMN 4260
LDSEHEESDE DVDMSDGMPD DLNKEEVGNE DEEVKQESGI ESDNENDEPG PEEDAGETET 4320
ALDEEEGAEE DVDMTNDEGK EDEENGPEEQ AMSDEEELKQ DAAMEENKEK GGEQNTEGLD 4380
GVEEKADTED IDQEAAVQQD SGSKGAGADA TDTQEQDDVG GSGTTQNTYE EDQEDVTKNN 4440
EESREEATAA LKQLGDSMKE YHRRRQDIKE AQTNGEEDEN LEKNNERPDE FEHVEGANTE 4500
TDTQALGSAT QDQLQTIDED MAIDDDREEQ EVDQKELVED ADDEKMDIDE EEMLSDIDAH 4560
DANNDVDSKK SGFIGKRKSE EDFENELSNE HFSADQEDDS EIQSLIENIE DNPPDASASL 4620
TPERSLEESR ELWHKSEIST ADLVSRLGEQ LRLILEPTLA TKLKGDYKTG KRLNMKRIIP 4680
YIASQFRKDK IWLRRTKPSK RQYQIMIALD DSKSMSESKC VKLAFDSLCL VSKTLTQLEA 4740
GGLSIVKFGE NIKEVHSFDQ QFSNESGARA FQWFGFQETK TDVKKLVAES TKIFERARAM 4800
VHNDQWQLEI VISDGICEDH ETIQKLVRRA RENKIMLVFV IIDGITSNES ILDMSQVNYI 4860
PDQYGNPQLK ITKYLDTFPF EFYVVVHDIS ELPEMLSLIL RQYFTDLASS 4910 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005654; C:nucleoplasm; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016887; F:ATPase activity; ISS:SGD.
 GO:0006461; P:protein complex assembly; IEA:InterPro.
 GO:0000027; P:ribosomal large subunit assembly; IMP:SGD.
 GO:0006364; P:rRNA processing; IMP:SGD. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR011704; ATPase_dyneun-rel_AAA.
 IPR012099; Midasin.
 IPR027417; P-loop_NTPase.
 IPR025662; Sigma_54_int_dom_ATP-bd_1.
 IPR002035; VWF_A. 
Pfam
 PF07728; AAA_5 
SMART
 SM00382; AAA
 SM00327; VWA 
PROSITE
 PS50234; VWFA 
PRINTS