CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-042852
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Trifunctional enzyme subunit alpha, mitochondrial 
Protein Synonyms/Alias
  
Gene Name
 HADHA 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
5***EKTSKDTSASAVacetylation[1]
5***EKTSKDTSASAVubiquitination[2]
26GKVIIVVKDGPGFYTacetylation[3]
55LQEGVDPKKLDSLTTacetylation[1, 3]
83EVGVDVAKHVAEDLGacetylation[1]
91HVAEDLGKVFGERFGubiquitination[4]
111LLTQMVSKGFLGRKSubiquitination[2, 4, 5, 6]
120FLGRKSGKGFYIYQEubiquitination[4]
130YIYQEGVKRKDLNSDacetylation[1, 3, 7]
130YIYQEGVKRKDLNSDubiquitination[4]
132YQEGVKRKDLNSDMDubiquitination[4]
243VDLYGAQKIVDRLKKacetylation[1, 3]
243VDLYGAQKIVDRLKKubiquitination[2, 4, 5, 7]
Reference
 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 250 AA 
Protein Sequence
EKTSKDTSAS AVAVGLKQGK VIIVVKDGPG FYTTRCLAPM MSEVIRILQE GVDPKKLDSL 60
TTSFGFPVGA ATLVDEVGVD VAKHVAEDLG KVFGERFGGG NPELLTQMVS KGFLGRKSGK 120
GFYIYQEGVK RKDLNSDMDS ILASLKLPPK SEVSSDEDIQ FRLVTRFVNE AVMCLQEGIL 180
ATPAEGDIGA VFGLGFPPCL GGWSRRLGRK LTLAWSPCCV SFNEVLFLYS GPFRFVDLYG 240
AQKIVDRLKK 250 
Gene Ontology
 GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:InterPro.
 GO:0050662; F:coenzyme binding; IEA:InterPro.
 GO:0006631; P:fatty acid metabolic process; IEA:InterPro. 
Interpro
 IPR006180; 3-OHacyl-CoA_DH_CS.
 IPR006108; 3HC_DH_C.
 IPR008927; 6-PGluconate_DH_C-like.
 IPR013328; DH_multihelical. 
Pfam
 PF00725; 3HCDH 
SMART
  
PROSITE
 PS00067; 3HCDH 
PRINTS