CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020167
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Acidic leucine-rich nuclear phosphoprotein 32 family member E 
Protein Synonyms/Alias
 LANP-like protein; LANP-L 
Gene Name
 ANP32E 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
5***MEMKKKINLELRubiquitination[1]
6**MEMKKKINLELRNubiquitination[1]
65ARLPSLNKLRKLELSubiquitination[1, 2, 3, 4, 5, 6, 7]
68PSLNKLRKLELSDNIubiquitination[1, 4, 8]
86GLEVLAEKCPNLTYLubiquitination[1]
99YLNLSGNKIKDLSTVubiquitination[1, 3, 4, 5, 6]
101NLSGNKIKDLSTVEAubiquitination[1, 2, 3, 4, 5, 7]
113VEALQNLKNLKSLDLubiquitination[1, 2, 3, 4, 5, 6, 7]
116LQNLKNLKSLDLFNCubiquitination[3, 8, 9]
254EGGLRGEKRKRDAEDubiquitination[3, 6]
256GLRGEKRKRDAEDDGsumoylation[10]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [9] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [10] Targeted identification of SUMOylation sites in human proteins using affinity enrichment and paralog-specific reporter ions.
 Lamoliatte F, Bonneil E, Durette C, Caron-Lizotte O, Wildemann D, Zerweck J, Wenschuh H, Thibault P.
 Mol Cell Proteomics. 2013 Jun 7;. [PMID: 23750026
Functional Description
 Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis process by modulating PP2A activity (By similarity). 
Sequence Annotation
 REPEAT 18 38 LRR 1.
 REPEAT 43 64 LRR 2.
 REPEAT 65 87 LRR 3.
 REPEAT 89 110 LRR 4.
 DOMAIN 123 161 LRRCT.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Leucine-rich repeat; Nucleus; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 268 AA 
Protein Sequence
MEMKKKINLE LRNRSPEEVT ELVLDNCLCV NGEIEGLNDT FKELEFLSMA NVELSSLARL 60
PSLNKLRKLE LSDNIISGGL EVLAEKCPNL TYLNLSGNKI KDLSTVEALQ NLKNLKSLDL 120
FNCEITNLED YRESIFELLQ QITYLDGFDQ EDNEAPDSEE EDDEDGDEDD EEEEENEAGP 180
PEGYEEEEEE EEEEDEDEDE DEDEAGSELG EGEEEVGLSY LMKEEIQDEE DDDDYVEEGE 240
EEEEEEEGGL RGEKRKRDAE DDGEEEDD 268 
Gene Ontology
 GO:0016023; C:cytoplasmic membrane-bounded vesicle; ISS:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0019212; F:phosphatase inhibitor activity; ISS:UniProtKB. 
Interpro
 IPR001611; Leu-rich_rpt.
 IPR025875; Leu-rich_rpt_4.
 IPR003603; U2A'_phosphoprotein32A_C. 
Pfam
 PF12799; LRR_4 
SMART
 SM00446; LRRcap 
PROSITE
 PS51450; LRR 
PRINTS