| Tag | Content |
|---|
| CPLM ID | CPLM-008793 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Cytoplasmic tRNA 2-thiolation protein 2 |
Protein Synonyms/Alias | Needs CLA4 to survive protein 2; Thiolation of uridine in cytoplasmic tRNA protein 2 |
Gene Name | NCS2 |
Gene Synonyms/Alias | CTU2; TUC2; YNL119W; N1913 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 362 | QPKSSMAKPSQCQIC | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation and in invasive and pseudohyphal growth. Inhibits replication of Brome mosaic virus. |
Sequence Annotation | MOD_RES 489 489 Phosphoserine. |
Keyword | Complete proteome; Cytoplasm; Phosphoprotein; Reference proteome; tRNA processing. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 493 AA |
Protein Sequence | MECQRCPASA RNPATVESRK EKFCDECFIK FVSTKQRKQM MKDEYFRNLF KVIYPFEKEG 60
SVSKILLPLS HSDSGSLVML DIVHDLLLEQ TKQHNNRTGF TVDVLTVFTE ENVSVIKERM 120
ESLINEKMSQ LNKISNIFNV HFIDVNEFFN NASEVSTFII DNENFEIFSK SKSVDDSNIL 180
TLKEILGKYC LNNSSRSDLI SIIKTQLIKH FAYENGYNAI MWGHSMTKLS EVIISLVVKG 240
KGSQIATFLD SESFDTLNNK PCKYKNLYPM KDLLSVEIES FLQIRNLAQF LINVEETNVK 300
PNCLIARKSL PSLGQQKLVK NMTINEITNK YFQDIQNDYS NIISTVLRTA DKLTQPKSSM 360
AKPSQCQICQ SKIYTNPSNW LNRITVTSPY PVETTEEKYL FKQWQDSKLG QSHTHYVELL 420
NEIKQGASNS LDVEDGDVKL CYGCLILLNT SIKDKNLVWP KVDTMDITAN ATNKNKELSQ 480
ILDQFEINSD GEE 493 |
Gene Ontology | GO:0005829; C:cytosol; IDA:UniProtKB. GO:0000049; F:tRNA binding; IMP:UniProtKB. GO:0001403; P:invasive growth in response to glucose limitation; IMP:SGD. GO:0032447; P:protein urmylation; IMP:SGD. GO:0007124; P:pseudohyphal growth; IMP:SGD. GO:0002143; P:tRNA wobble position uridine thiolation; IMP:SGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |