CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-041817
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Pyruvate kinase 
Protein Synonyms/Alias
  
Gene Name
 Pklr 
Gene Synonyms/Alias
 mCG_17567 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
158VAIALDTKGPEIRTGubiquitination[1]
190VLVTVDPKFRTRGDAacetylation[2]
190VLVTVDPKFRTRGDAubiquitination[1]
230LISLVVRKIGPEGLVubiquitination[1]
250GGFLGNRKGVNLPNAubiquitination[1]
419SFPVEAVKMQHAIARubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Glycolysis; Kinase; Magnesium; Pyruvate; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 574 AA 
Protein Sequence
MSVQENELPQ QLWPWIFKSQ KDLAKSALSG APGGPAGYLR RASVAQLTQE LGTAFFQQQQ 60
LPAAMADTFL EHLCLLDIDS EPVAARSTSI IATIGPASRS VDRLKEMIKA GMNIARLNFS 120
HGSHEYHAES IANIREAAES FATSPLSYRP VAIALDTKGP EIRTGVLQGG PESEVEIVKG 180
SQVLVTVDPK FRTRGDAKTV WVDYHNITQV VAVGGRIYID DGLISLVVRK IGPEGLVTEV 240
EHGGFLGNRK GVNLPNAEVD LPGLSEQDLL DLRFGVEHNV DIIFASFVRK ASDVVAVRDA 300
LGPEGRGIKI ISKIENHEGV KKFDEILEVS DGIMVARGDL GIEIPAEKVF LAQKMMIGRC 360
NLAGKPVVCA TQMLESMITK ARPTRAETSD VANAVLDGAD CIMLSGETAK GSFPVEAVKM 420
QHAIAREAEA AVYHRQLFEE LRRAAPLSRD PTEVTAIGAV EASFKCCAAA IIVLTKTGRS 480
AQLLSRYRPR AAVIAVTRSA QAARQVHLSR GVFPLLYREP PEAVWADDVD RRVQFGIESG 540
KLRGFLRVGD LVIVVTGWRP GSGYTNIMRV LTIS 574 
Gene Ontology
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0030955; F:potassium ion binding; IEA:InterPro.
 GO:0004743; F:pyruvate kinase activity; IDA:MGI.
 GO:0006754; P:ATP biosynthetic process; IEA:Compara.
 GO:0032869; P:cellular response to insulin stimulus; IEA:Compara.
 GO:0006096; P:glycolysis; IEA:UniProtKB-UniPathway.
 GO:0042866; P:pyruvate biosynthetic process; IEA:Compara.
 GO:0033198; P:response to ATP; IEA:Compara.
 GO:0051591; P:response to cAMP; IEA:Compara.
 GO:0009749; P:response to glucose stimulus; IEA:Compara.
 GO:0009408; P:response to heat; IEA:Compara.
 GO:0001666; P:response to hypoxia; IEA:Compara.
 GO:0010226; P:response to lithium ion; IEA:Compara.
 GO:0007584; P:response to nutrient; IEA:Compara.
 GO:0051707; P:response to other organism; IMP:MGI. 
Interpro
 IPR001697; Pyr_Knase.
 IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
 IPR011037; Pyrv_Knase-like_insert_dom.
 IPR015794; Pyrv_Knase_a/b.
 IPR018209; Pyrv_Knase_AS.
 IPR015793; Pyrv_Knase_brl.
 IPR015795; Pyrv_Knase_C.
 IPR015806; Pyrv_Knase_insert_dom. 
Pfam
 PF00224; PK
 PF02887; PK_C 
SMART
  
PROSITE
 PS00110; PYRUVATE_KINASE 
PRINTS
 PR01050; PYRUVTKNASE.