CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021478
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Splicing factor, arginine/serine-rich 19 
Protein Synonyms/Alias
 SR-related and CTD-associated factor 1; SR-related-CTD-associated factor; SCAF; Serine arginine-rich pre-mRNA splicing factor SR-A1; SR-A1 
Gene Name
 SCAF1 
Gene Synonyms/Alias
 SFRS19; SRA1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
55QGDLPNDKDGSRCHGubiquitination[1, 2, 3, 4, 5]
158LRAWRTGKTVSPQSNubiquitination[5]
865GLGSIGVKFSRDRESubiquitination[4]
1123ANLASRAKAQELIQAubiquitination[4, 5, 6, 7, 8]
1226EEVKLAIKPYYQKKDubiquitination[5, 8]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 May function in pre-mRNA splicing (By similarity). 
Sequence Annotation
 REGION 1187 1312 Necessary for interaction with the CTD
 MOD_RES 239 239 Phosphoserine.
 MOD_RES 335 335 Phosphothreonine (By similarity).
 MOD_RES 448 448 Phosphoserine.
 MOD_RES 453 453 Phosphoserine.
 MOD_RES 498 498 Phosphoserine.
 MOD_RES 500 500 Phosphoserine.
 MOD_RES 526 526 Phosphoserine.
 MOD_RES 537 537 Phosphoserine.
 MOD_RES 548 548 Phosphoserine.
 MOD_RES 552 552 Phosphoserine.
 MOD_RES 612 612 Phosphoserine.
 MOD_RES 614 614 Phosphoserine.
 MOD_RES 734 734 Phosphoserine.
 MOD_RES 738 738 Phosphoserine.
 MOD_RES 874 874 Phosphoserine.
 MOD_RES 929 929 Phosphoserine.
 MOD_RES 936 936 Phosphoserine.
 MOD_RES 965 965 Phosphoserine.
 MOD_RES 976 976 Phosphothreonine.
 MOD_RES 989 989 Phosphothreonine.
 MOD_RES 1001 1001 Phosphothreonine.  
Keyword
 Complete proteome; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1312 AA 
Protein Sequence
MEEEDESRGK TEESGEDRGD GPPDRDPTLS PSAFILRAIQ QAVGSSLQGD LPNDKDGSRC 60
HGLRWRRCRS PRSEPRSQES GGTDTATVLD MATDSFLAGL VSVLDPPDTW VPSRLDLRPG 120
ESEDMLELVA EVRIGDRDPI PLPVPSLLPR LRAWRTGKTV SPQSNSSRPT CARHLTLGTG 180
DGGPAPPPAP SSASSSPSPS PSSSSPSPPP PPPPPAPPAP PAPRFDIYDP FHPTDEAYSP 240
PPAPEQKYDP FEPTGSNPSS SAGTPSPEEE EEEEEEEEEE EEDEEEEEGL SQSISRISET 300
LAGIYDDNSL SQDFPGDESP RPDAQPTQPT PAPGTPPQVD STRADGAMRR RVFVVGTEAE 360
ACREGKVSVE VVTAGGAALP PPLLPPGDSE IEEGEIVQPE EEPRLALSLF RPGGRAARPT 420
PAASATPTAQ PLPQPPAPRA PEGDDFLSLH AESDGEGALQ VDLGEPAPAP PAADSRWGGL 480
DLRRKILTQR RERYRQRSPS PAPAPAPAAA AGPPTRKKSR RERKRSGEAK EAASSSSGTQ 540
PAPPAPASPW DSKKHRSRDR KPGSHASSSA RRRSRSRSRS RSTRRRSRST DRRRGGSRRS 600
RSREKRRRRR RSASPPPATS SSSSSRRERH RGKHRDGGGS KKKKKRSRSR GEKRSGDGSE 660
KAPAPAPPPS GSTSCGDRDS RRRGAVPPSI QDLTDHDLFA IKRTITVGRL DKSDPRGPSP 720
APASSPKREV LYDSEGLSGE ERGGKSSQKD RRRSGAASSS SSSREKGSRR KALDGGDRDR 780
DRDRDRDRDR SSKKARPPKE SAPSSGPPPK PPVSSGSGSS SSSSSCSSRK VKLQSKVAVL 840
IREGVSSTTP AKDAASAGLG SIGVKFSRDR ESRSPFLKPD ERAPTEMAKA APGSTKPKKT 900
KVKAKAGAKK TKGTKGKTKP SKTRKKVRSG GGSGGSGGQV SLKKSKADSC SQAAGTKGAE 960
ETSWSGEERA AKVPSTPPPK AAPPPPALTP DSQTVDSSCK TPEVSFLPEE ATEEAGVRGG 1020
AEEEEEEEEE EEEEEEEEEQ QPATTTATST AAAAPSTAPS AGSTAGDSGA EDGPASRVSQ 1080
LPTLPPPMPW NLPAGVDCTT SGVLALTALL FKMEEANLAS RAKAQELIQA TNQILSHRKP 1140
PSSLGMTPAP VPTSLGLPPG PSSYLLPGSL PLGGCGSTPP TPTGLAATSD KREGSSSSEG 1200
RGDTDKYLKK LHTQERAVEE VKLAIKPYYQ KKDITKEEYK DILRKAVHKI CHSKSGEINP 1260
VKVSNLVRAY VQRYRYFRKH GRKPGDPPGP PRPPKEPGPP DKGGPGLPLP PL 1312 
Gene Ontology
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
 GO:0006366; P:transcription from RNA polymerase II promoter; IEA:Compara. 
Interpro
  
Pfam
  
SMART
  
PROSITE
  
PRINTS