UniProt Accession

 ITPA_YEAST; P47119; D6VWP0 

Genbank Protein ID

 Z49569; L47993; AY557897; BK006943 

Genbank Nucleotide ID

 CAA89597.1; AAB39295.1; AAS56223.1; DAA08856.1 

Protein Name

 Inosine triphosphate pyrophosphatase 

Protein Synonyms/Alias

 ITPase; Inosine triphosphatase; Hydroxylaminopurine sensitivity protein 1; Non-canonical purine NTP pyrophosphatase; Non-standard purine NTP pyrophosphatase; Nucleoside-triphosphate diphosphatase; Nucleoside-triphosphate pyrophosphatase; NTPase 

Gene Name

 HAM1 

Gene Synonyms/Alias

 YJR069C; J1811 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
60	AIALAKGKQAVAALG	ubiquitination	[1]
97	AYIKWFLKSMGLEKI	acetylation	[2]
106	MGLEKIVKMLEPFEN	acetylation	[2]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Pyrophosphatase that hydrolyzes the non-canonical purine nucleotides inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triposphate (dHAPTP) and 5-bromodeoxyuridine 5'-triphosphate (BrdUTP) to their respective monophosphate derivatives. Xanthosine 5'-triphosphate (XTP) is also a potential substrate. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions. 

Sequence Annotation

 REGION 10 15 Substrate binding (By similarity).
 REGION 76 77 Substrate binding (By similarity).
 REGION 151 154 Substrate binding (By similarity).
 REGION 180 181 Substrate binding (By similarity).
 METAL 45 45 Magnesium or manganese (By similarity).
 METAL 76 76 Magnesium or manganese (By similarity).
 BINDING 58 58 Substrate (By similarity).
 BINDING 175 175 Substrate (By similarity).  

Keyword

 Complete proteome; Cytoplasm; Hydrolase; Magnesium; Manganese; Metal-binding; Nucleotide metabolism; Nucleotide-binding; Nucleus; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 197 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0008828; F:dATP pyrophosphohydrolase activity; IDA:SGD.
 GO:0047840; F:dCTP diphosphatase activity; IDA:SGD.
 GO:0036217; F:dGTP diphosphatase activity; IDA:SGD.
 GO:0035870; F:dITP diphosphatase activity; IDA:SGD.
 GO:0036218; F:dTTP diphosphatase activity; IDA:SGD.
 GO:0004170; F:dUTP diphosphatase activity; IDA:SGD.
 GO:0036219; F:GTP diphosphatase activity; IDA:SGD.
 GO:0036220; F:ITP diphosphatase activity; IDA:SGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
 GO:0036221; F:UTP diphosphatase activity; IDA:SGD.
 GO:0036222; F:XTP diphosphatase activity; IDA:SGD.
 GO:0009117; P:nucleotide metabolic process; IEA:UniProtKB-KW.
 GO:0009217; P:purine deoxyribonucleoside triphosphate catabolic process; IDA:SGD.
 GO:0009213; P:pyrimidine deoxyribonucleoside triphosphate catabolic process; IDA:SGD. 

Interpro

 IPR002637; Ham1p-like.
 IPR027502; ITPase. 

Pfam

 PF01725; Ham1p_like 

SMART

  

PROSITE

  

PRINTS