CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009678
UniProt Accession
Genbank Protein ID
 M64300 
Genbank Nucleotide ID
Protein Name
 Mitogen-activated protein kinase 1 
Protein Synonyms/Alias
 MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2 
Gene Name
 Mapk1 
Gene Synonyms/Alias
 Erk2; Mapk; Prkm1 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
268YLLSLPHKNKVPWNRacetylation[1]
283LFPNADSKALDLLDKacetylation[1]
290KALDLLDKMLTFNPHacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in respons to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation (By similarity). 
Sequence Annotation
 DOMAIN 23 311 Protein kinase.
 NP_BIND 29 37 ATP.
 REGION 103 109 Inhibitor-binding.
 MOTIF 183 185 TXY.
 ACT_SITE 147 147 Proton acceptor.
 BINDING 52 52 ATP.
 BINDING 106 106 Inhibitor; via amide nitrogen and
 BINDING 164 164 Inhibitor.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 27 27 Phosphoserine; by SGK1 (By similarity).
 MOD_RES 183 183 Phosphothreonine; by MAP2K1 and MAP2K2
 MOD_RES 185 185 Phosphotyrosine; by MAP2K1 and MAP2K2 (By
 MOD_RES 188 188 Phosphothreonine; by autocatalysis.
 MOD_RES 244 244 Phosphoserine (By similarity).
 MOD_RES 246 246 Phosphoserine (By similarity).
 MOD_RES 282 282 Phosphoserine (By similarity).  
Keyword
 3D-structure; Acetylation; Apoptosis; ATP-binding; Cell cycle; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 358 AA 
Protein Sequence
MAAAAAAGPE MVRGQVFDVG PRYTNLSYIG EGAYGMVCSA YDNLNKVRVA IKKISPFEHQ 60
TYCQRTLREI KILLRFRHEN IIGINDIIRA PTIEQMKDVY IVQDLMETDL YKLLKTQHLS 120
NDHICYFLYQ ILRGLKYIHS ANVLHRDLKP SNLLLNTTCD LKICDFGLAR VADPDHDHTG 180
FLTEYVATRW YRAPEIMLNS KGYTKSIDIW SVGCILAEML SNRPIFPGKH YLDQLNHILG 240
ILGSPSQEDL NCIINLKARN YLLSLPHKNK VPWNRLFPNA DSKALDLLDK MLTFNPHKRI 300
EVEQALAHPY LEQYYDPSDE PIAEAPFKFD MELDDLPKEK LKELIFEETA RFQPGYRS 358 
Gene Ontology
 GO:0005901; C:caveola; TAS:UniProtKB.
 GO:0005856; C:cytoskeleton; TAS:UniProtKB.
 GO:0005829; C:cytosol; TAS:UniProtKB.
 GO:0032839; C:dendrite cytoplasm; IDA:RGD.
 GO:0005769; C:early endosome; TAS:UniProtKB.
 GO:0005925; C:focal adhesion; TAS:UniProtKB.
 GO:0005794; C:Golgi apparatus; TAS:UniProtKB.
 GO:0005770; C:late endosome; TAS:UniProtKB.
 GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; TAS:UniProtKB.
 GO:0005654; C:nucleoplasm; IDA:UniProtKB.
 GO:0043204; C:perikaryon; IDA:RGD.
 GO:0043234; C:protein complex; IDA:RGD.
 GO:0031143; C:pseudopodium; IEA:Compara.
 GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IDA:RGD.
 GO:0004707; F:MAP kinase activity; IDA:UniProtKB.
 GO:0008353; F:RNA polymerase II carboxy-terminal domain kinase activity; IEA:Compara.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0050853; P:B cell receptor signaling pathway; IEA:Compara.
 GO:0072584; P:caveolin-mediated endocytosis; TAS:UniProtKB.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0019858; P:cytosine metabolic process; IEA:Compara.
 GO:0070371; P:ERK1 and ERK2 cascade; IEA:Compara.
 GO:0060716; P:labyrinthine layer blood vessel development; IEA:Compara.
 GO:0031663; P:lipopolysaccharide-mediated signaling pathway; IEA:Compara.
 GO:0033598; P:mammary gland epithelial cell proliferation; IEA:Compara.
 GO:0000189; P:MAPK import into nucleus; IDA:RGD.
 GO:0045596; P:negative regulation of cell differentiation; IEA:Compara.
 GO:0009887; P:organ morphogenesis; IEA:Compara.
 GO:0018105; P:peptidyl-serine phosphorylation; IDA:MGI.
 GO:0030335; P:positive regulation of cell migration; IEP:RGD.
 GO:0008284; P:positive regulation of cell proliferation; IEP:RGD.
 GO:0010800; P:positive regulation of peptidyl-threonine phosphorylation; IEA:Compara.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IEP:RGD.
 GO:0045727; P:positive regulation of translation; IMP:RGD.
 GO:0051493; P:regulation of cytoskeleton organization; TAS:UniProtKB.
 GO:2000641; P:regulation of early endosome to late endosome transport; TAS:UniProtKB.
 GO:0090170; P:regulation of Golgi inheritance; TAS:UniProtKB.
 GO:0032872; P:regulation of stress-activated MAPK cascade; TAS:UniProtKB.
 GO:0006974; P:response to DNA damage stimulus; IEA:Compara.
 GO:0070849; P:response to epidermal growth factor stimulus; ISS:UniProtKB.
 GO:0043627; P:response to estrogen stimulus; IDA:RGD.
 GO:0043330; P:response to exogenous dsRNA; IEA:Compara.
 GO:0009636; P:response to toxic substance; IDA:RGD.
 GO:0019233; P:sensory perception of pain; IMP:UniProtKB.
 GO:0050852; P:T cell receptor signaling pathway; IEA:Compara. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR003527; MAP_kinase_CS.
 IPR008349; MAPK_ERK1/2.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS01351; MAPK
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS
 PR01770; ERK1ERK2MAPK.