UniProt Accession

 SUCA_HUMAN; P53597; Q9BWB0; Q9UNP6 

Genbank Protein ID

 AC096770; BC000504; AF104921; Z68204 

Genbank Nucleotide ID

 AAH00504.1; AAD17940.2; CAA92426.1 

Protein Name

 Succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial 

Protein Synonyms/Alias

 Succinyl-CoA synthetase subunit alpha; SCS-alpha 

Gene Name

 SUCLG1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
54	RQHLYVDKNTKIICQ	acetylation	[1]
66	ICQGFTGKQGTFHSQ	ubiquitination	[2, 3]
105	LPVFNTVKEAKEQTG	ubiquitination	[4]
308	GAIIAGGKGGAKEKI	ubiquitination	[4]
312	AGGKGGAKEKISALQ	ubiquitination	[5]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Catalyzes the ATP- or GTP-dependent ligation of succinate and CoA to form succinyl-CoA. The nature of the beta subunit determines the nucleotide specificity (By similarity). 

Sequence Annotation

 ACT_SITE 299 299 Tele-phosphohistidine intermediate (By
 MOD_RES 54 54 N6-acetyllysine.
 CROSSLNK 280 280 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; Complete proteome; Disease mutation; GTP-binding; Isopeptide bond; Ligase; Mitochondrion; Nucleotide-binding; Reference proteome; Transit peptide; Tricarboxylic acid cycle; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 346 AA 

Protein Sequence

Gene Ontology

 GO:0005743; C:mitochondrial inner membrane; IEA:Compara.
 GO:0005759; C:mitochondrial matrix; TAS:Reactome.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0045244; C:succinate-CoA ligase complex (GDP-forming); IEA:Compara.
 GO:0003878; F:ATP citrate synthase activity; IEA:InterPro.
 GO:0048037; F:cofactor binding; IEA:InterPro.
 GO:0019003; F:GDP binding; IEA:Compara.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0004775; F:succinate-CoA ligase (ADP-forming) activity; IEA:EC.
 GO:0004776; F:succinate-CoA ligase (GDP-forming) activity; IEA:EC.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0006105; P:succinate metabolic process; IEA:Compara.
 GO:0006104; P:succinyl-CoA metabolic process; IEA:Compara.
 GO:0006099; P:tricarboxylic acid cycle; TAS:Reactome. 

Interpro

 IPR017440; Cit_synth/succinyl-CoA_lig_AS.
 IPR003781; CoA-bd.
 IPR005810; CoA_lig_alpha.
 IPR005811; CoA_ligase.
 IPR016040; NAD(P)-bd_dom.
 IPR016102; Succinyl-CoA_synth-like. 

Pfam

 PF02629; CoA_binding
 PF00549; Ligase_CoA 

SMART

 SM00881; CoA_binding 

PROSITE

 PS01216; SUCCINYL_COA_LIG_1
 PS00399; SUCCINYL_COA_LIG_2 

PRINTS

 PR01798; SCOASYNTHASE.