UniProt Accession

 OTC1_ECOLI; P04391; Q2M655 

Genbank Protein ID

 J02842; X00210; U14003; U00096; AP009048 

Genbank Nucleotide ID

 AAA23483.1; CAA25037.1; AAA97150.1; AAC77211.1; BAE78251.1 

Protein Name

 Ornithine carbamoyltransferase chain I 

Protein Synonyms/Alias

 OTCase-1 

Gene Name

 argI 

Gene Synonyms/Alias

 b4254; JW4211 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
42	KSGKEEAKLTGKNIA	acetylation	[1]
54	NIALIFEKDSTRTRC	acetylation	[1]
87	SGSQIGHKESIKDTA	acetylation	[1]
91	IGHKESIKDTARVLG	acetylation	[1]
259	REYQVNSKMMQLTGN	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline, which is a substrate for argininosuccinate synthetase, the enzyme involved in the final step in arginine biosynthesis. 

Sequence Annotation

 REGION 56 60 Carbamoyl phosphate binding.
 REGION 134 137 Carbamoyl phosphate binding.
 REGION 236 237 Ornithine binding.
 REGION 273 276 Carbamoyl phosphate binding.
 METAL 274 274 Zinc.
 BINDING 10 10 Carbamoyl phosphate (By similarity).
 BINDING 72 72 Carbamoyl phosphate (By similarity).
 BINDING 83 83 Carbamoyl phosphate.
 BINDING 107 107 Carbamoyl phosphate.
 BINDING 168 168 Ornithine.
 BINDING 232 232 Ornithine.
 BINDING 302 302 Carbamoyl phosphate (By similarity).
 BINDING 320 320 Carbamoyl phosphate.  

Keyword

 3D-structure; Allosteric enzyme; Amino-acid biosynthesis; Arginine biosynthesis; Complete proteome; Cytoplasm; Direct protein sequencing; Metal-binding; Reference proteome; Transferase; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 334 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016597; F:amino acid binding; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004585; F:ornithine carbamoyltransferase activity; IDA:EcoCyc.
 GO:0006526; P:arginine biosynthetic process; IEA:HAMAP. 

Interpro

 IPR006132; Asp/Orn_carbamoyltranf_P-bd.
 IPR006130; Asp/Orn_carbamoylTrfase.
 IPR006131; Asp_carbamoyltransf_Asp/Orn-bd.
 IPR002292; Orn/put_carbamltrans.
 IPR024904; Orn_carbamltrans. 

Pfam

 PF00185; OTCace
 PF02729; OTCace_N 

SMART

  

PROSITE

 PS00097; CARBAMOYLTRANSFERASE 

PRINTS

 PR00100; AOTCASE.
 PR00102; OTCASE.