UniProt Accession

 DPB4_YEAST; Q04603; D6VSA7 

Genbank Protein ID

 Z48758; AY557673; BK006938 

Genbank Nucleotide ID

 CAA88674.1; AAS55999.1; DAA11967.1 

Protein Name

 DNA polymerase epsilon subunit D 

Protein Synonyms/Alias

 DNA polymerase II subunit D 

Gene Name

 DPB4 

Gene Synonyms/Alias

 YDR121W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
114	LKGPVRDKLDEYQAA	ubiquitination	[1]
177	DVEESSEKKQKTESQ	acetylation	[2]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 DNA polymerase epsilon (DNA polymerase II) participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair. Also functions as component of the ISW2 complex, which acts in remodeling the chromatin by catalyzing an ATP-dependent alteration in the structure of nucleosomal DNA. THe ISW2 complex is involved in coordinating transcriptional repression and in inheritance of telomeric silencing. It is involved in repression of MAT a- specific genes, INO1, and early meiotic genes during mitotic growth dependent upon transcription factor UME6 and in a parallel pathway to the RPD3-SIN3 histone deacetylase complex. 

Sequence Annotation

 MOD_RES 183 183 Phosphoserine; by ATM or ATR.  

Keyword

 Complete proteome; Direct protein sequencing; DNA replication; DNA-directed DNA polymerase; Nucleotidyltransferase; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 196 AA 

Protein Sequence

Gene Ontology

 GO:0008623; C:CHRAC; IDA:SGD.
 GO:0008622; C:epsilon DNA polymerase complex; IDA:SGD.
 GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
 GO:0031492; F:nucleosomal DNA binding; IDA:SGD.
 GO:0043044; P:ATP-dependent chromatin remodeling; IDA:SGD.
 GO:0042276; P:error-prone translesion synthesis; IDA:SGD.
 GO:0070868; P:heterochromatin organization involved in chromatin silencing; IMP:SGD.
 GO:0006272; P:leading strand elongation; IMP:SGD.
 GO:0042766; P:nucleosome mobilization; IDA:SGD.
 GO:0090399; P:replicative senescence; IMP:SGD.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR009072; Histone-fold. 

Pfam

  

SMART

  

PROSITE

  

PRINTS