CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037898
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Poly(U)-binding-splicing factor PUF60 
Protein Synonyms/Alias
  
Gene Name
 PUF60 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
225DLSDDDIKSVFEAFGubiquitination[1, 2, 3, 4]
233SVFEAFGKIKSCTLAacetylation[5]
233SVFEAFGKIKSCTLAubiquitination[6]
235FEAFGKIKSCTLARDubiquitination[2]
249DPTTGKHKGYGFIEYubiquitination[2, 3]
283GQYLRVGKAVTPPMPubiquitination[1, 3]
Reference
 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 323 AA 
Protein Sequence
MATATIALVN GQQGGGSEPA AAAAVVAAGD KWKPPQGTDS IKMENGQSTA AKLGLPPLTP 60
EQQEALQKAK KYAMEQSIKS VLVKQTIAHQ QQQLTNLQMA AQRQRALAIM CRVYVGSIYY 120
ELGEDTIRQA FAPFGPIKSI DMSWDSVTMK HKGFAFVEYE VPEAAQLALE QMNSVMLGGR 180
NIKVGRPSNI GQAQPIIDQL AEEARAFNRI YVASVHQDLS DDDIKSVFEA FGKIKSCTLA 240
RDPTTGKHKG YGFIEYEKAQ SSQDAVSSMN LFDLGGQYLR VGKAVTPPMP LLTPATPGGL 300
PPAAAVAAAA ATAKITAQVR AHT 323 
Gene Ontology
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0000166; F:nucleotide binding; IEA:InterPro. 
Interpro
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR006532; PolyU-bd.
 IPR000504; RRM_dom. 
Pfam
 PF00076; RRM_1 
SMART
 SM00360; RRM 
PROSITE
 PS50102; RRM 
PRINTS