CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005604
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcription elongation factor SPT5 
Protein Synonyms/Alias
 Chromatin elongation factor SPT5 
Gene Name
 SPT5 
Gene Synonyms/Alias
 YML010W; YM9571.08 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
69PPLDGEEKEAKSEPQubiquitination[1]
317NLDRAMGKKKLKILSacetylation[2]
318LDRAMGKKKLKILSIacetylation[2]
341RIYIEAPKQSVIEKFubiquitination[1]
424VPRLDYGKFDEIDPTacetylation[3]
466LDQANLYKRDDRHFTubiquitination[1]
770SNKMDLSKMNPEIISacetylation[3]
778MNPEIISKMGPPSSKacetylation[3]
785KMGPPSSKTFQQPIQubiquitination[1]
803GREVALGKTVRIRSAacetylation[2, 3]
820KGQLGIVKDVNGDKAacetylation[3]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] mChIP-KAT-MS, a method to map protein interactions and acetylation sites for lysine acetyltransferases.
 Mitchell L, Huard S, Cotrut M, Pourhanifeh-Lemeri R, Steunou AL, Hamza A, Lambert JP, Zhou H, Ning Z, Basu A, Côté J, Figeys DA, Baetz K.
 Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1641-50. [PMID: 23572591]
 [3] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 The SPT4-SPT5 complex mediates both activation and inhibition of transcription elongation, and plays a role in pre- mRNA processing. This complex seems to be important for the stability of the RNA polymerase II elongation machinery on the chromatin template but not for the inherent ability of this machinery to translocate down the gene. 
Sequence Annotation
 DOMAIN 382 415 KOW 1.
 DOMAIN 533 560 KOW 2.
 DOMAIN 584 615 KOW 3.
 DOMAIN 799 832 KOW 4.
 REPEAT 931 936 1.
 REPEAT 937 942 2.
 REPEAT 948 953 3.
 REPEAT 958 963 4.
 REPEAT 969 974 5.
 REPEAT 975 980 6.
 REPEAT 981 986 7.
 REPEAT 987 992 8.
 REPEAT 1000 1005 9.
 REPEAT 1009 1014 10.
 REPEAT 1015 1020 11.
 REPEAT 1032 1037 12.
 REPEAT 1043 1048 13.
 REPEAT 1052 1057 14.
 REPEAT 1058 1063 15.
 REGION 931 1063 15 X 6 AA tandem repeats of S-[TA]-W-G-G-
 MOD_RES 35 35 Phosphothreonine.
 MOD_RES 133 133 Phosphothreonine.
 MOD_RES 136 136 Phosphoserine.
 MOD_RES 188 188 Phosphoserine.
 MOD_RES 219 219 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; Mitochondrion; mRNA processing; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1063 AA 
Protein Sequence
MSDNSDTNVS MQDHDQQFAD PVVVPQSTDT KDENTSDKDT VDSGNVTTTE STERAESTSN 60
IPPLDGEEKE AKSEPQQPED NAETAATEQV SSSNGPATDD AQATLNTDSS EANEIVKKEE 120
GSDERKRPRE EDTKNSDGDT KDEGDNKDED DDEDDDDDDD DEDDDDEAPT KRRRQERNRF 180
LDIEAEVSDD EDEDEDEEDS ELVREGFITH GDDEDDEASA PGARRDDRLH RQLDQDLNKT 240
SEEDAQRLAK ELRERYGRSS SKQYRAAAQD GYVPQRFLLP SVDTATIWGV RCRPGKEKEL 300
IRKLLKKKFN LDRAMGKKKL KILSIFQRDN YTGRIYIEAP KQSVIEKFCN GVPDIYISQK 360
LLIPVQELPL LLKPNKSDDV ALEEGSYVRI KRGIYKGDLA MVDQISENNL EVMLKIVPRL 420
DYGKFDEIDP TTQQRKSRRP TFAHRAPPQL FNPTMALRLD QANLYKRDDR HFTYKNEDYI 480
DGYLYKSFRI QHVETKNIQP TVEELARFGS KEGAVDLTSV SQSIKKAQAA KVTFQPGDRI 540
EVLNGEQRGS KGIVTRTTKD IATIKLNGFT TPLEFPISTL RKIFEPGDHV TVINGEHQGD 600
AGLVLMVEQG QVTFMSTQTS REVTITANNL SKSIDTTATS SEYALHDIVE LSAKNVACII 660
QAGHDIFKVI DETGKVSTIT KGSILSKINT ARARVSSVDA NGNEIKIGDT IVEKVGSRRE 720
GQVLYIQTQQ IFVVSKKIVE NAGVFVVNPS NVEAVASKDN MLSNKMDLSK MNPEIISKMG 780
PPSSKTFQQP IQSRGGREVA LGKTVRIRSA GYKGQLGIVK DVNGDKATVE LHSKNKHITI 840
DKHKLTYYNR EGGEGITYDE LVNRRGRVPQ ARMGPSYVSA PRNMATGGIA AGAAATSSGL 900
SGGMTPGWSS FDGGKTPAVN AHGGSGGGGV SSWGGASTWG GQGNGGASAW GGAGGGASAW 960
GGQGTGATST WGGASAWGNK SSWGGASTWA SGGESNGAMS TWGGTGDRSA YGGASTWGGN 1020
NNNKSTRDGG ASAWGNQDDG NRSAWNNQGN KSNYGGNSTW GGH 1063 
Gene Ontology
 GO:0032044; C:DSIF complex; IPI:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0001181; F:core RNA polymerase I binding transcription factor activity; IC:SGD.
 GO:0000991; F:core RNA polymerase II binding transcription factor activity; IMP:SGD.
 GO:0019843; F:rRNA binding; IDA:SGD.
 GO:0006370; P:7-methylguanosine mRNA capping; IGI:SGD.
 GO:0008298; P:intracellular mRNA localization; IMP:SGD.
 GO:0000398; P:mRNA splicing, via spliceosome; IMP:SGD.
 GO:2001208; P:negative regulation of transcription elongation from RNA polymerase I promoter; IGI:SGD.
 GO:2001209; P:positive regulation of transcription elongation from RNA polymerase I promoter; IMP:SGD.
 GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IMP:SGD.
 GO:2000232; P:regulation of rRNA processing; IMP:SGD.
 GO:0090262; P:regulation of transcription-coupled nucleotide-excision repair; IGI:SGD. 
Interpro
 IPR005824; KOW.
 IPR024945; Spt5_C_dom.
 IPR022581; Spt5_N.
 IPR017071; TF_Spt5.
 IPR005100; TF_Spt5_NGN-domain.
 IPR006645; Transcrpt_antiterm_NusG_N.
 IPR008991; Translation_prot_SH3-like. 
Pfam
 PF00467; KOW
 PF03439; Spt5-NGN
 PF11942; Spt5_N 
SMART
 SM01104; CTD
 SM00739; KOW
 SM00738; NGN 
PROSITE
  
PRINTS