UniProt Accession

 NDK_YEAST; P36010; D6VXM0 

Genbank Protein ID

 D13562; X75780; Z28067; AY558263; BK006944 

Genbank Nucleotide ID

 BAA02758.1; CAA53407.1; CAA81904.1; AAS56589.1; DAA09090.1 

Protein Name

 Nucleoside diphosphate kinase 

Protein Synonyms/Alias

 NDK; NDP kinase 

Gene Name

 YNK1 

Gene Synonyms/Alias

 NDK1; YNK; YKL067W; YKL333 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
13	ERTFIAVKPDGVQRG	ubiquitination	[1]
47	KLVKADDKLLEQHYA	acetylation	[2]
59	HYAEHVGKPFFPKMV	acetylation	[2]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Required for repair of UV radiation- and etoposide-induced DNA damage. 

Sequence Annotation

 ACT_SITE 119 119 Pros-phosphohistidine intermediate (By
 BINDING 13 13 ATP (By similarity).
 BINDING 61 61 ATP (By similarity).
 BINDING 89 89 ATP (By similarity).
 BINDING 95 95 ATP (By similarity).
 BINDING 106 106 ATP (By similarity).
 BINDING 116 116 ATP (By similarity).
 MOD_RES 95 95 Phosphothreonine.  

Keyword

 3D-structure; ATP-binding; Complete proteome; Cytoplasm; DNA damage; DNA repair; Kinase; Magnesium; Metal-binding; Mitochondrion; Nucleotide metabolism; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 153 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:SGD.
 GO:0005758; C:mitochondrial intermembrane space; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004550; F:nucleoside diphosphate kinase activity; IDA:SGD.
 GO:0006241; P:CTP biosynthetic process; IEA:InterPro.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0006183; P:GTP biosynthetic process; IEA:InterPro.
 GO:0009142; P:nucleoside triphosphate biosynthetic process; IDA:SGD.
 GO:0006974; P:response to DNA damage stimulus; IMP:SGD.
 GO:0006228; P:UTP biosynthetic process; IEA:InterPro. 

Interpro

 IPR001564; Nucleoside_diP_kinase.
 IPR023005; Nucleoside_diP_kinase_AS. 

Pfam

 PF00334; NDK 

SMART

 SM00562; NDK 

PROSITE

 PS00469; NDP_KINASES 

PRINTS

 PR01243; NUCDPKINASE.