UniProt Accession

 B5MC51_HUMAN; B5MC51 

Genbank Protein ID

 AC002073 

Genbank Nucleotide ID

  

Protein Name

 LIM domain kinase 2 

Protein Synonyms/Alias

  

Gene Name

 LIMK2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
194	ALSTLDTKENLEGTL	ubiquitination	[1]
214	RRSNSISKSPGPSSP	ubiquitination	[1]
222	SPGPSSPKEPLLFSR	ubiquitination	[1]
269	GFFGQAIKVTHKATG	ubiquitination	[1]
277	VTHKATGKVMVMKEL	ubiquitination	[1]
282	TGKVMVMKELIRCDE	ubiquitination	[1]
293	RCDEETQKTFLTEVK	ubiquitination	[1]
300	KTFLTEVKVMRSLDH	ubiquitination	[1, 2]
321	IGVLYKDKKLNLLTE	ubiquitination	[1]
350	DPFPWQQKVRFAKGI	ubiquitination	[1]
596	DAWASRVKELLVDCY	ubiquitination	[1, 2, 3]
604	ELLVDCYKPTEAFIS	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 

Functional Description

  

Sequence Annotation

  

Keyword

 ATP-binding; Complete proteome; LIM domain; Metal-binding; Nucleotide-binding; Reference proteome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 629 AA 

Protein Sequence

Gene Ontology

 GO:0005801; C:cis-Golgi network; IEA:Compara.
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:Compara.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0042325; P:regulation of phosphorylation; IEA:InterPro.
 GO:0007283; P:spermatogenesis; IEA:Compara. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR001478; PDZ.
 IPR008025; PP1_inhibitor.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR001781; Znf_LIM. 

Pfam

 PF00412; LIM
 PF00595; PDZ
 PF00069; Pkinase
 PF05361; PP1_inhibitor 

SMART

 SM00132; LIM
 SM00228; PDZ 

PROSITE

 PS50023; LIM_DOMAIN_2
 PS50106; PDZ
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM 

PRINTS