CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005100
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Alpha-1-antitrypsin 1-2 
Protein Synonyms/Alias
 AAT; Alpha-1 protease inhibitor 2; Alpha-1-antiproteinase; Serine protease inhibitor 1-2; Serine protease inhibitor A1b; Serpin A1b 
Gene Name
 Serpina1b 
Gene Synonyms/Alias
 Aat2; Dom2; Spi1-2 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
186VEKGTQGKIVEAVKEubiquitination[1]
214LFKGKWKKPFDPENTacetylation[2]
287HLEQTLNKELISKILubiquitination[1]
292LNKELISKILLNRRRacetylation[2, 3, 4, 5]
292LNKELISKILLNRRRubiquitination[1]
318ISGDYNLKTLMSPLGubiquitination[1]
347TEENAPLKLSKAVHKubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [3] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [4] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [5] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 
Functional Description
 Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. 
Sequence Annotation
 REGION 368 387 RCL.
 CARBOHYD 64 64 N-linked (GlcNAc...) (Potential).
 CARBOHYD 101 101 N-linked (GlcNAc...).
 CARBOHYD 265 265 N-linked (GlcNAc...).  
Keyword
 Acute phase; Complete proteome; Glycoprotein; Protease inhibitor; Reference proteome; Secreted; Serine protease inhibitor; Signal. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 413 AA 
Protein Sequence
MTPSISWGLL LLAGLCCMVP SFLAEDVQET DTSQKDQSPA SHEIATNLGD FAISLYRELV 60
HQSNTSNIFF SPVSIATAFA MLSLGSKGDT HTQILEGLQF NLTQTSEADI HKSFQHLLQT 120
LNRPDSELQL STGNGLFVNN DLKLVEKFLE EAKNHYQAEV FSVNFAESEE AKKVINDFVE 180
KGTQGKIVEA VKELDQDTVF ALANYILFKG KWKKPFDPEN TEEAEFHVDK STTVKVPMMM 240
LSGMLDVHHC SILSSWVLLM DYAGNASAVF LLPEDGKMQH LEQTLNKELI SKILLNRRRR 300
LVQIHIPRLS ISGDYNLKTL MSPLGITRIF NNGADLSGIT EENAPLKLSK AVHKAVLTID 360
ETGTEAAAAT VFEAVPMSMP PILRFDHPFL FIIFEEHTQS PIFVGKVVDP THK 413 
Gene Ontology
 GO:0005576; C:extracellular region; IDA:MGI.
 GO:0004867; F:serine-type endopeptidase inhibitor activity; IMP:MGI.
 GO:0006953; P:acute-phase response; IEA:UniProtKB-KW.
 GO:0001701; P:in utero embryonic development; IMP:MGI.
 GO:0006487; P:protein N-linked glycosylation; IDA:MGI.
 GO:0030162; P:regulation of proteolysis; IBA:RefGenome.
 GO:0034097; P:response to cytokine stimulus; IDA:MGI.
 GO:0043434; P:response to peptide hormone stimulus; IDA:MGI. 
Interpro
 IPR023795; Protease_inhib_I4_serpin_CS.
 IPR023796; Serpin_dom.
 IPR000215; Serpin_fam. 
Pfam
 PF00079; Serpin 
SMART
 SM00093; SERPIN 
PROSITE
 PS00284; SERPIN 
PRINTS